2bo6

From Proteopedia

Revision as of 08:01, 30 October 2007

DISSECTION OF MANNOSYLGLYCERATE SYNTHASE: AN ARCHETYPAL MANNOSYLTRANSFERASE

Overview

The enzymatic transfer of activated mannose yields mannosides in, glycoconjugates and oligo- and polysaccharides. Yet, despite its, biological necessity, the mechanism by which glycosyltransferases, recognize mannose and catalyze its transfer to acceptor molecules is, poorly understood. Here, we report broad high-throughput screening and, kinetic analyses of both natural and synthetic substrates of Rhodothermus, marinus mannosylglycerate synthase (MGS), which catalyzes the formation of, the stress protectant 2-O-alpha-D-mannosyl glycerate. The sequence of MGS, indicates that it is at the cusp of inverting and retaining transferases., The structures of apo MGS and complexes with donor and acceptor molecules, including GDP-mannose, combined with mutagenesis of the binding and, catalytic ... [(full description)]

About this Structure

2BO6 is a [Single protein] structure of sequence from [Rhodothermus marinus] with MN and DGY as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural dissection and high-throughput screening of mannosylglycerate synthase., Flint J, Taylor E, Yang M, Bolam DN, Tailford LE, Martinez-Fleites C, Dodson EJ, Davis BG, Gilbert HJ, Davies GJ, Nat Struct Mol Biol. 2005 Jul;12(7):608-14. Epub 2005 Jun 12. PMID:15951819

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