1boi
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1BOI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]]. Active as [[http://en.wikipedia.org/wiki/ | + | 1BOI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]]. Active as [[http://en.wikipedia.org/wiki/Thiosulfate_sulfurtransferase Thiosulfate sulfurtransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.1 2.8.1.1]]. Structure known Active Site: CT1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOI OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Thiosulfate sulfurtransferase]] | ||
[[Category: Berni, R.]] | [[Category: Berni, R.]] | ||
[[Category: Cianci, M.]] | [[Category: Cianci, M.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:07:37 2007'' |
Revision as of 08:02, 30 October 2007
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N-TERMINALLY TRUNCATED RHODANESE
Overview
The NH2-terminal sequence of rhodanese influences many of its properties, ranging from mitochondrial import to folding. Rhodanese truncated by >9, residues is degraded in Escherichia coli. Mutant enzymes with lesser, truncations are recoverable and active, but they show altered active site, reactivities (Trevino, R. J., Tsalkova, T., Dramer, G., Hardesty, B., Chirgwin, J. M., and Horowitz, P. M. (1998) J. Biol. Chem. 273, 27841-27847), suggesting that the NH2-terminal sequence stabilizes the, overall structure. We tested aspects of the conformations of these, shortened species. Intrinsic and probe fluorescence showed that truncation, decreased stability and increased hydrophobic exposure, while near UV CD, suggested altered tertiary structure. Under native conditions, truncated, ... [(full description)]
About this Structure
1BOI is a [Single protein] structure of sequence from [Bos taurus]. Active as [Thiosulfate sulfurtransferase], with EC number [2.8.1.1]. Structure known Active Site: CT1. Full crystallographic information is available from [OCA].
Reference
NH2-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions., Trevino RJ, Gliubich F, Berni R, Cianci M, Chirgwin JM, Zanotti G, Horowitz PM, J Biol Chem. 1999 May 14;274(20):13938-47. PMID:10318804
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