364d

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spinqualitylabelsall models 364d, resolution 3.000Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3.0 A STRUCTURE OF FRAGMENT I FROM E. COLI 5S RRNA

Overview

Two new RNA structures portray how non-Watson-Crick base pairs and metal, ions can produce a unique RNA shape suitable for recognition by proteins., The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a, duplex dodecamer encompassing an internal loop E have been determined at, 3.0 and 1.5 A, respectively. This loop E region is distorted by three, "cross-strand purine stacks" and three novel, water-mediated noncanonical, base pairs and stabilized by a four metal ion zipper. These features give, its minor groove a unique hydrogen-bonding surface and make the adjacent, major groove wide enough to permit recognition by the ribosomal protein, L25, which is expected to bind to this surface.

About this Structure

364D is a Protein complex structure of sequences from [1] with MG as ligand. This structure superseeds the now removed PDB entry 356D. Full crystallographic information is available from OCA.

Reference

Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain., Correll CC, Freeborn B, Moore PB, Steitz TA, Cell. 1997 Nov 28;91(5):705-12. PMID:9393863 [[Category: ]]

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