2asi

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==About this Structure==
==About this Structure==
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2ASI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]] with NAG as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1ASI. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.23 3.4.23.23]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ASI OCA]].
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2ASI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]] with NAG as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1ASI. Active as [[http://en.wikipedia.org/wiki/Mucorpepsin Mucorpepsin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.23 3.4.23.23]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ASI OCA]].
==Reference==
==Reference==
Crystal structure of the aspartic proteinase from Rhizomucor miehei at 2.15 A resolution., Yang J, Teplyakov A, Quail JW, J Mol Biol. 1997 May 2;268(2):449-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9159482 9159482]
Crystal structure of the aspartic proteinase from Rhizomucor miehei at 2.15 A resolution., Yang J, Teplyakov A, Quail JW, J Mol Biol. 1997 May 2;268(2):449-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9159482 9159482]
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[[Category: Mucorpepsin]]
[[Category: Rhizomucor miehei]]
[[Category: Rhizomucor miehei]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: zymogen]]
[[Category: zymogen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:25:41 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:09:44 2007''

Revision as of 08:05, 30 October 2007


2asi, resolution 2.15Å

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ASPARTIC PROTEINASE

Overview

The crystal structure of the aspartic proteinase from Rhizomucor miehei, (RMP, EC 3. 4. 23. 23) has been refined to 2.15 A resolution to a, crystallographic R-value of 0.215 and an Rfree of 0.281. The, root-mean-square (r.m.s.) error for the atomic coordinates estimated from, a Luzzati plot is 0.2 A. The r.m.s. deviations for the bond distances and, bond angles from ideality are 0.01 A and 1.7 degrees, respectively. RMP, contains two domains that consist predominantly of beta-sheets. A large, substrate-binding cleft is clearly visible between the two domains, and, the two catalytic residues Asp38 and Asp237 are located in the middle of, the cleft with a water molecule bridging the carboxyl groups of Asp38 and, Asp237. Due to crystal packing, the C-terminal domain is more mobile than, the ... [(full description)]

About this Structure

2ASI is a [Single protein] structure of sequence from [Rhizomucor miehei] with NAG as [ligand]. This structure superseeds the now removed PDB entry 1ASI. Active as [Mucorpepsin], with EC number [3.4.23.23]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].

Reference

Crystal structure of the aspartic proteinase from Rhizomucor miehei at 2.15 A resolution., Yang J, Teplyakov A, Quail JW, J Mol Biol. 1997 May 2;268(2):449-59. PMID:9159482

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