1aa6

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[[Image:1aa6.gif|left|200px]]<br />
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[[Image:1aa6.gif|left|200px]]<br /><applet load="1aa6" size="450" color="white" frame="true" align="right" spinBox="true"
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<applet load="1aa6" size="450" color="white" frame="true" align="right" spinBox="true"
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caption="1aa6, resolution 2.3&Aring;" />
caption="1aa6, resolution 2.3&Aring;" />
'''REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br />
'''REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br />
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==About this Structure==
==About this Structure==
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1AA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SF4, MGD and 4MO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Structure known Active Sites: 4MO and FS4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA].
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1AA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SF4, MGD and 4MO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Known structural/functional Sites: <scene name='pdbsite=4MO:The Mo Atom Is Coordinated To The Se Atom Of Sec 140 And ...'>4MO</scene> and <scene name='pdbsite=FS4:The Fe S Cluster Is Coordinated To The S Atoms Of CYS 8, ...'>FS4</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA].
==Reference==
==Reference==
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[[Category: selenocysteine]]
[[Category: selenocysteine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:46:14 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:11:16 2007''

Revision as of 12:01, 18 December 2007

Image:1aa6.gif

1aa6, resolution 2.3Å

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REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI

Overview

Formate dehydrogenase H from Escherichia coli contains selenocysteine, (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD), cofactors, and an Fe4S4 cluster at the active site and catalyzes the, two-electron oxidation of formate to carbon dioxide. The crystal, structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate, dehydrogenase H (with and without bound inhibitor) and the reduced, [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain, alphabeta structure with the molybdenum directly coordinated to selenium, and both MGD cofactors. These structures suggest a reaction mechanism that, directly involves SeCys140 and His141 in proton abstraction and the, molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron, transfer.

About this Structure

1AA6 is a Single protein structure of sequence from Escherichia coli with SF4, MGD and 4MO as ligands. Active as Formate dehydrogenase, with EC number 1.2.1.2 Known structural/functional Sites: and . Full crystallographic information is available from OCA.

Reference

Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855

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