1awz
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1awz. | + | [[Image:1awz.jpg|left|200px]]<br /><applet load="1awz" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1awz" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1awz" /> | caption="1awz" /> | ||
'''3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES'''<br /> | '''3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1AWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. | + | 1AWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=NLS:Nucleolar Localization Signal'>NLS</scene>, <scene name='pdbsite=RBS:Putative Cell Receptor Binding Site'>RBS</scene> and <scene name='pdbsite=RIB:Ribonucleolytic Active Site'>RIB</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AWZ OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 24: | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:22:39 2007'' |
Revision as of 12:12, 18 December 2007
|
3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES
Contents |
Overview
Human angiogenin is a member of the pancreatic ribonuclease superfamily, that induces blood vessel formation. Its three-dimensional solution, structure has been determined to high resolution by heteronuclear NMR, spectroscopy. 30 structures were calculated, based on a total of 1441, assigned NOE correlations, 64 coupling constants and 50 hydrogen bonds., The backbone atomic rms difference from the mean coordinates is 0.067 +/-, 0.012 nm and 0.13 nm from the previously determined crystal structure. The, side-chain of Gln117 was found to obstruct the active site as observed in, the crystal state. There was no evidence of an alternative open form of, angiogenin, although two sets of chemical shifts were observed for some, residues, mainly around the active site and in the C-terminal segment. The, topology of the ribonucleolytic active site is described with a particular, emphasis on the conformation and protonation of active-site His residues., The side-chain of His114 adopts two main conformations in solution. In, contrast to pancreatic ribonuclease A, His13 was shown to be more basic, than His114, with pKa values of 6.65 and 6.05 respectively. The His47, residue is located in an environment very resistant to protonation with a, pKa lower than 4.
Disease
Known diseases associated with this structure: Amyotrophic lateral sclerosis, susceptibility to OMIM:[105850]
About this Structure
1AWZ is a Single protein structure of sequence from Homo sapiens. Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values., Lequin O, Thuring H, Robin M, Lallemand JY, Eur J Biochem. 1997 Dec 15;250(3):712-26. PMID:9461294
Page seeded by OCA on Tue Dec 18 14:22:39 2007
