3pcn
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 3PCN is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with FE, BME and DHY as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ | + | 3PCN is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with FE, BME and DHY as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Protocatechuate_3,4-dioxygenase Protocatechuate 3,4-dioxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.3 1.13.11.3]]. Structure known Active Sites: ACA, ACB, ACC, ACD, ACE, ACF, VEA, VEB, VEC, VED, VEE and VEF. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3PCN OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure and resonance Raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate., Elgren TE, Orville AM, Kelly KA, Lipscomb JD, Ohlendorf DH, Que L Jr, Biochemistry. 1997 Sep 23;36(38):11504-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9298971 9298971] | Crystal structure and resonance Raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate., Elgren TE, Orville AM, Kelly KA, Lipscomb JD, Ohlendorf DH, Que L Jr, Biochemistry. 1997 Sep 23;36(38):11504-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9298971 9298971] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| + | [[Category: Protocatechuate 3,4-dioxygenase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Lipscomb, J.D.]] | [[Category: Lipscomb, J.D.]] | ||
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[[Category: substrate complex]] | [[Category: substrate complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:21:38 2007'' |
Revision as of 08:16, 30 October 2007
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STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3,4-DIHYDROXYPHENYLACETATE
Overview
The crystal structure of the anaerobic complex of Pseudomonas putida, protocatechuate 3,4-dioxygenase (3,4-PCD) bound with the alternative, substrate, 3,4-dihydroxyphenylacetate (HPCA), is reported at 2.4 A, resolution and refined to an R factor of 0.17. Formation of the active, site Fe(III).HPCA chelated complex causes the endogenous axial tyrosinate, Tyr447 (147beta), to dissociate from the iron and rotate into an, alternative orientation analogous to that previously observed in the, anaerobic 3,4-PCD.3,4-dihydroxybenzoate complex (3, 4-PCD.PCA) [Orville, A. M., Lipscomb, J. D., & Ohlendorf, D. H. (1997) Biochemistry 36, 10052-10066]. Two orientations of the aromatic ring of HPCA related by an, approximate 180 degrees rotation within the active site are consistent, with the electron ... [(full description)]
About this Structure
3PCN is a [Protein complex] structure of sequences from [Pseudomonas putida] with FE, BME and DHY as [ligands]. Active as [Protocatechuate 3,4-dioxygenase], with EC number [1.13.11.3]. Structure known Active Sites: ACA, ACB, ACC, ACD, ACE, ACF, VEA, VEB, VEC, VED, VEE and VEF. Full crystallographic information is available from [OCA].
Reference
Crystal structure and resonance Raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate., Elgren TE, Orville AM, Kelly KA, Lipscomb JD, Ohlendorf DH, Que L Jr, Biochemistry. 1997 Sep 23;36(38):11504-13. PMID:9298971
Page seeded by OCA on Tue Oct 30 10:21:38 2007
