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1bqy
From Proteopedia
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| - | [[Image:1bqy.gif|left|200px]]<br /> | + | [[Image:1bqy.gif|left|200px]]<br /><applet load="1bqy" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1bqy" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1bqy, resolution 2.50Å" /> | caption="1bqy, resolution 2.50Å" /> | ||
'''PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM'''<br /> | '''PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BQY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viridovipera_stejnegeri Viridovipera stejnegeri]. | + | 1BQY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viridovipera_stejnegeri Viridovipera stejnegeri]. Known structural/functional Sites: <scene name='pdbsite=A:Catalytic Sites'>A</scene> and <scene name='pdbsite=B:Catalytic Sites'>B</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BQY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: snake venom]] | [[Category: snake venom]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:31:54 2007'' |
Revision as of 12:22, 18 December 2007
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PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM
Overview
BACKGROUND: Trimeresurus stejnejeri venom plasminogen activator (TSV-PA), is a snake venom serine proteinase that specifically activates, plasminogen. Snake venom serine proteinases form a subfamily of, trypsin-like proteinases that are characterised by a high substrate, specificity and resistance to inhibition. Many of these venom enzymes, specifically interfere with haemostatic mechanisms and display a long, circulating half-life. For these reasons several of them have commercial, applications and are potentially attractive pharmacological tools., RESULTS: The crystal structure of TSV-PA has been determined to 2.5 A, resolution and refined to an R factor of 17.8 (R free, 24.4). The enzyme, showing the overall polypeptide fold of trypsin-like serine proteinases, displays unique structural elements such as the presence of a, phenylalanine at position 193, a C-terminal tail clamped via a disulphide, bridge to the 99-loop, and a structurally conserved Asp97 residue. The, presence of a cis proline at position 218 is in agreement with, evolutionary relationships to glandular kallikrein. CONCLUSIONS: We, postulate that Phe 193 accounts for the high substrate specificity of, TSV-PA and renders it incapable of forming a stable complex with bovine, pancreatic trypsin inhibitor and other extended substrates and inhibitors., Mutational studies previously showed that Asp97 is crucial for the, plasminogenolytic activity of TSV-PA, here we identify the conservation of, Asp97 in both types of mammalian plasminogen activator - tissue-type (tPA), and urokinase-type (uPA). It seems likely that Asp97 of tPA and uPA will, have a similar role in plasminogen recognition. The C-terminal extension, of TSV-PA is conserved among snake venom serine proteinases, although its, function is unknown. The three-dimensional structure presented here is the, first of a snake venom serine proteinase and provides an excellent, template for modelling other homologous family members.
About this Structure
1BQY is a Single protein structure of sequence from Viridovipera stejnegeri. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
The crystal structure of the novel snake venom plasminogen activator TSV-PA: a prototype structure for snake venom serine proteinases., Parry MA, Jacob U, Huber R, Wisner A, Bon C, Bode W, Structure. 1998 Sep 15;6(9):1195-206. PMID:9753698
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