1c89
From Proteopedia
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| - | [[Image:1c89.gif|left|200px]]<br /> | + | [[Image:1c89.gif|left|200px]]<br /><applet load="1c89" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1c89" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1c89" /> | caption="1c89" /> | ||
'''NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES'''<br /> | '''NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1C89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]. | + | 1C89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]. Known structural/functional Sites: <scene name='pdbsite=IC1:Ice Binding Site'>IC1</scene> and <scene name='pdbsite=IC2:Ice Binding Site'>IC2</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thermal hysteresis protein]] | [[Category: thermal hysteresis protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:36:33 2007'' |
Revision as of 12:26, 18 December 2007
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NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES
Overview
The structure of a new antifreeze protein (AFP) variant, RD3, from, antarctic eel pout (Rhigophila dearborni) with enhanced activity has been, determined for the first time by nuclear magnetic resonance spectroscopy., RD3 comprises a unique translational topology of two homologous type III, AFP globular domains, each containing one flat, ice binding plane. The ice, binding plane of the N domain is located approximately 3.5 A "behind" that, of the C domain. The two ice binding planes are located laterally with an, angle of 32 +/- 12 degrees between the planes. These results suggest that, the C domain plane of RD3 binds first to the ice [1010] prism plane in the, <0001> direction, which induces successive ice binding of the N domain in, the <0101> direction. This manner of ice binding caused by the unique, structural topology of RD3 is thought to be crucial for the significant, enhancement of antifreeze activity, especially at low AFP concentrations.
About this Structure
1C89 is a Single protein structure of sequence from Pachycara brachycephalum. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity., Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S, J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:11010977
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