1dyt
From Proteopedia
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| - | [[Image:1dyt.gif|left|200px]]<br /> | + | [[Image:1dyt.gif|left|200px]]<br /><applet load="1dyt" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1dyt" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1dyt, resolution 1.75Å" /> | caption="1dyt, resolution 1.75Å" /> | ||
'''X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A'''<br /> | '''X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DYT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with FE and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1DYT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with FE and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=ASA:Potential Active Site Chain A'>ASA</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DYT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rnase 3]] | [[Category: rnase 3]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:47:11 2007'' |
Revision as of 12:37, 18 December 2007
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X-RAY CRYSTAL STRUCTURE OF ECP (RNASE 3) AT 1.75 A
Overview
Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found, only in eosinophil leukocytes that belongs to the RNase A superfamily., This enzyme is bactericidal, helminthotoxic and cytotoxic to mammalian, cells and tissues. The protein has been cloned, heterologously, overexpressed, purified and crystallized. Its crystal structure has been, determined and refined using data up to 1. 75 A resolution. The molecule, displays the alpha+beta folding topology typical for members of the, ribonuclease A superfamily. The catalytic active site residues are, conserved with respect to other ribonucleases of the superfamily but some, differences appear at substrate recognition subsites, which may account, in part, for the low catalytic activity. Most strikingly, 19, surface-located arginine residues confer a strong basic character to the, protein. The high concentration of positive charges and the particular, orientation of the side-chains of these residues may also be related to, the low activity of ECP as a ribonuclease and provides an explanation for, its unique cytotoxic role through cell membrane disruption.
About this Structure
1DYT is a Single protein structure of sequence from Homo sapiens with FE and CIT as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 A resolution., Mallorqui-Fernandez G, Pous J, Peracaula R, Aymami J, Maeda T, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Ruth FX, Coll M, J Mol Biol. 2000 Jul 28;300(5):1297-307. PMID:10903870
Page seeded by OCA on Tue Dec 18 14:47:11 2007
Categories: Homo sapiens | Single protein | Coll, M. | Gomis-Rueth, F.X. | Llorens, R.De. | Maeda, T. | Mallorqui-Fernandez, G. | Peracaula, R. | Pous, J. | Seno, M. | Tada, H. | Yamada, H. | CIT | FE | Ecp | Eosinophil cationic protein | Rnase 3
