1e42
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1e42.gif|left|200px]]<br /> | + | [[Image:1e42.gif|left|200px]]<br /><applet load="1e42" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e42" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1e42, resolution 1.70Å" /> | caption="1e42, resolution 1.70Å" /> | ||
'''BETA2-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2'''<br /> | '''BETA2-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1E42 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL, MG, NI, DTD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1E42 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL, MG, NI, DTD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Mo5 Binding Site For Chain A Symmetry Related Subunits C ...'>AC1</scene>, <scene name='pdbsite=AC2:Mo5 Binding Site For Chain B Symmetry Related Subunits C ...'>AC2</scene>, <scene name='pdbsite=AC3:Mo5 Binding Site For Chain B Symmetry Related Subunits C ...'>AC3</scene>, <scene name='pdbsite=AC4:Mo5 Binding Site For Chain B Symmetry Related Subunits C ...'>AC4</scene> and <scene name='pdbsite=AC5:Nik Binding Site For Chain B Symmetry Related Subunits C ...'>AC5</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E42 OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 24: | ||
[[Category: endocytosis]] | [[Category: endocytosis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:54:08 2007'' |
Revision as of 12:44, 18 December 2007
|
BETA2-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2
Overview
The heterotetrameric AP2 adaptor (alpha, beta 2, mu 2 and sigma 2, subunits) plays a central role in clathrin-mediated endocytosis. We, present the protein recruitment function and 1.7 A resolution structure of, its beta 2-appendage domain to complement those previously determined for, the mu 2 subunit and alpha appendage. Using structure-directed, mutagenesis, we demonstrate the ability of the beta 2 appendage alone to, bind directly to clathrin and the accessory proteins AP180, epsin and, eps15 at the same site. Clathrin polymerization is promoted by binding of, clathrin simultaneously to the beta 2-appendage site and to a second site, on the adjacent beta 2 hinge. This results in the displacement of the, other ligands from the beta 2 appendage. Thus clathrin binding to an, AP2-accessory protein complex would cause the controlled release of, accessory proteins at sites of vesicle formation.
About this Structure
1E42 is a Single protein structure of sequence from Homo sapiens with CL, MG, NI, DTD and GOL as ligands. Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.
Reference
The structure and function of the beta 2-adaptin appendage domain., Owen DJ, Vallis Y, Pearse BM, McMahon HT, Evans PR, EMBO J. 2000 Aug 15;19(16):4216-27. PMID:10944104
Page seeded by OCA on Tue Dec 18 14:54:08 2007
Categories: Homo sapiens | Single protein | Evans, P.R. | Mcmahon, H.T. | Owen, D.J. | CL | DTD | GOL | MG | NI | Adaptor | Endocytosis
