This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1e9r
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1e9r. | + | [[Image:1e9r.jpg|left|200px]]<br /><applet load="1e9r" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e9r" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1e9r, resolution 2.40Å" /> | caption="1e9r, resolution 2.40Å" /> | ||
'''BACTERIAL CONJUGATIVE COUPLING PROTEIN TRWBDELTAN70. TRIGONAL FORM IN COMPLEX WITH SULPHATE.'''<br /> | '''BACTERIAL CONJUGATIVE COUPLING PROTEIN TRWBDELTAN70. TRIGONAL FORM IN COMPLEX WITH SULPHATE.'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1E9R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1E9R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=SO1:So4 Binding Site For Residue G602 Nucleotide-Binding Sit ...'>SO1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9R OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 24: | ||
[[Category: ring helicases]] | [[Category: ring helicases]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:07:05 2007'' |
Revision as of 12:57, 18 December 2007
|
BACTERIAL CONJUGATIVE COUPLING PROTEIN TRWBDELTAN70. TRIGONAL FORM IN COMPLEX WITH SULPHATE.
Overview
The transfer of DNA across membranes and between cells is a central, biological process; however, its molecular mechanism remains unknown. In, prokaryotes, trans-membrane passage by bacterial conjugation, is the main, route for horizontal gene transfer. It is the means for rapid acquisition, of new genetic information, including antibiotic resistance by pathogens., Trans-kingdom gene transfer from bacteria to plants or fungi and even, bacterial sporulation are special cases of conjugation. An integral, membrane DNA-binding protein, called TrwB in the Escherichia coli R388, conjugative system, is essential for the conjugation process. This large, multimeric protein is responsible for recruiting the relaxosome, DNA-protein complex, and participates in the transfer of a single DNA, strand during cell mating. Here we report the three-dimensional structure, of a soluble variant of TrwB. The molecule consists of two domains: a, nucleotide-binding domain of alpha/beta topology, reminiscent of RecA and, DNA ring helicases, and an all-alpha domain. Six equivalent protein, monomers associate to form an almost spherical quaternary structure that, is strikingly similar to F1-ATPase. A central channel, 20 A in width, traverses the hexamer.
About this Structure
1E9R is a Single protein structure of sequence from Escherichia coli with SO4 as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase., Gomis-Ruth FX, Moncalian G, Perez-Luque R, Gonzalez A, Cabezon E, de la Cruz F, Coll M, Nature. 2001 Feb 1;409(6820):637-41. PMID:11214325
Page seeded by OCA on Tue Dec 18 15:07:05 2007
