User:Marc-Michael Blum/Sandbox1

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== Testing Sandbox ==
== Testing Sandbox ==
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<applet scene='User:Marc-Michael_Blum/Sandbox1/2gvw_rainbow/2' size='300' frame='true' align='right' caption='Tertiary structure of DFPase with two calcium ions shown as green spheres' />
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<applet scene='User:Marc-Michael_Blum/Sandbox1/2gvw_rainbow/2' size='300' frame='true' align='right' caption='Tertiary structure of DFPase with the two calcium ions shown as green spheres' />
Lorem ipsum dolor sit amet, consectetur adipiscing elit<ref>PMID:11162408</ref>. Pellentesque tincidunt rutrum sem eu luctus. Phasellus malesuada scelerisque tortor, at consequat nulla ullamcorper ac. Quisque luctus metus sed purus malesuada porta. Proin sed dui dignissim risus auctor imperdiet a ac lectus. Aliquam erat volutpat. Aenean augue purus, tempor at dapibus id, ultrices ut purus. Vivamus ut risus odio, id consectetur justo. Phasellus ullamcorper risus vitae purus convallis ac interdum dui tincidunt. Donec nunc ligula, placerat lobortis rutrum vel, ultrices nec lectus. Donec tortor est, gravida a volutpat nec, suscipit et enim. Sed ultricies nunc eu mauris sodales nec condimentum risus pellentesque. Etiam elementum erat eget dui pretium quis eleifend ante auctor.
Lorem ipsum dolor sit amet, consectetur adipiscing elit<ref>PMID:11162408</ref>. Pellentesque tincidunt rutrum sem eu luctus. Phasellus malesuada scelerisque tortor, at consequat nulla ullamcorper ac. Quisque luctus metus sed purus malesuada porta. Proin sed dui dignissim risus auctor imperdiet a ac lectus. Aliquam erat volutpat. Aenean augue purus, tempor at dapibus id, ultrices ut purus. Vivamus ut risus odio, id consectetur justo. Phasellus ullamcorper risus vitae purus convallis ac interdum dui tincidunt. Donec nunc ligula, placerat lobortis rutrum vel, ultrices nec lectus. Donec tortor est, gravida a volutpat nec, suscipit et enim. Sed ultricies nunc eu mauris sodales nec condimentum risus pellentesque. Etiam elementum erat eget dui pretium quis eleifend ante auctor.

Revision as of 16:12, 30 March 2010

Testing Sandbox

Tertiary structure of DFPase with the two calcium ions shown as green spheres

Drag the structure with the mouse to rotate

Lorem ipsum dolor sit amet, consectetur adipiscing elit[1]. Pellentesque tincidunt rutrum sem eu luctus. Phasellus malesuada scelerisque tortor, at consequat nulla ullamcorper ac. Quisque luctus metus sed purus malesuada porta. Proin sed dui dignissim risus auctor imperdiet a ac lectus. Aliquam erat volutpat. Aenean augue purus, tempor at dapibus id, ultrices ut purus. Vivamus ut risus odio, id consectetur justo. Phasellus ullamcorper risus vitae purus convallis ac interdum dui tincidunt. Donec nunc ligula, placerat lobortis rutrum vel, ultrices nec lectus. Donec tortor est, gravida a volutpat nec, suscipit et enim. Sed ultricies nunc eu mauris sodales nec condimentum risus pellentesque. Etiam elementum erat eget dui pretium quis eleifend ante auctor.

Aenean dui odio, aliquet eget congue sit amet, lobortis et ante. Suspendisse interdum iaculis eros id mattis. Pellentesque euismod ante vitae mauris auctor non adipiscing arcu dignissim. Nunc ac nisi dolor. Nam blandit aliquet mauris, porta mollis velit volutpat vel. Phasellus sed libero eget arcu rutrum faucibus. Etiam sollicitudin, erat id ullamcorper tempor, urna ligula dictum orci, id fermentum metus diam ac massa. Nulla nisl massa, varius vulputate iaculis ac, tempus non magna. Sed auctor faucibus blandit. Nunc quis arcu quis arcu pharetra tristique nec ullamcorper leo. Integer porta, arcu at volutpat mattis, ante nisi faucibus velit, vitae molestie lacus eros vitae eros. Suspendisse nec blandit elit. Nam magna sapien, tempor eu condimentum non, tincidunt nec ante. Proin ut nisi ipsum. Cras pretium sapien in augue iaculis nec semper lacus lacinia. Donec lectus neque, commodo eget vehicula eu, venenatis vitae nunc. Proin tempus hendrerit eleifend. Sed sit amet nisl vitae tortor consequat vestibulum. Integer iaculis scelerisque eleifend.

3D Structures of squid DFPase

  • 1e1a This is the original structure solved at 1.8 Å (cryo conditions)
  • 1pjx Atomic resolution structure of DFPase solved at 0.85 Å.
  • 2gvw Structure solved at at 1.86 Å (room temperature).
  • 3byc This is the neutron structure of DFPase solved by joint X-ray and neutron refinement.
  • 3kgg X-ray structure of perdeuterated DFPase at 2.1 Å (room temperature).
  • 2gvv DFPase in complex with dicyclopentylphosphoroamidate (DcPPA)
  • 3li3 DFPase mutant D121E
  • 3li4 DFPase mutant N120D/N175D/D229N
  • 3li5 DFPase mutant E21Q/N120D/N175D/D229N
  • 3hlh DFPase mutant E37A/Y144A/R146A/T195M
  • 3hli DFPase mutant E37D/Y144A/R146A/T195M
  • 2gvu DFPase mutant D229N/N120D
  • 2gvx DFPase mutant D229N/N175D
  • 2iax DFPase mutant D232S
  • 2iaw DFPase mutant D175D
  • 2iav DFPase mutant H287A
  • 2iau DFPase mutant W244Y
  • 2iat DFPase mutant W244L
  • 2ias DFPase mutant W244F
  • 2iar DFPase mutant W244H
  • 2iaq DFPase mutant S271A
  • 2iap DFPase mutant E21Q
  • 2iao DFPase mutant E37Q

References & Notes

  1. ↑ Hartleib J, Ruterjans H. High-yield expression, purification, and characterization of the recombinant diisopropylfluorophosphatase from Loligo vulgaris. Protein Expr Purif. 2001 Feb;21(1):210-9. PMID:11162408 doi:10.1006/prep.2000.1360

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Marc-Michael Blum

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