1gw0
From Proteopedia
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| - | [[Image:1gw0. | + | [[Image:1gw0.jpg|left|200px]]<br /><applet load="1gw0" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1gw0" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1gw0, resolution 2.4Å" /> | caption="1gw0, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM'''<br /> | '''CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GW0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces] with NAG, CU, CL, SO4 and OXY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] | + | 1GW0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces] with NAG, CU, CL, SO4 and OXY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GW0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxygen reduction]] | [[Category: oxygen reduction]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:40:51 2007'' |
Revision as of 13:31, 18 December 2007
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CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM
Overview
We have crystallized the ascomycete laccase from Melanocarpus albomyces, with all four coppers present and determined the crystal structure at 2.4, A resolution. The enzyme is heavily glycosylated and consists of three, cupredoxin-like domains, similar to those found in the Cu-depleted, basidiomycete laccase from Coprinus cinereus. However, there are, significant differences in the loops forming the substrate-binding pocket., In addition, the crystal structure of the M. albomyces laccase revealed, elongated electron density between all three coppers in the trinuclear, copper site, suggesting that an oxygen molecule binds with a novel, geometry. This oxygen, required in the reaction, may enter the trinuclear, site through the tunnel, which is open in the structure of the C. cinereus, laccase. In contrast, the C-terminus on the M. albomyces laccase forms a, plug that blocks this access.
About this Structure
1GW0 is a Single protein structure of sequence from Melanocarpus albomyces with NAG, CU, CL, SO4 and OXY as ligands. Active as Laccase, with EC number 1.10.3.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site., Hakulinen N, Kiiskinen LL, Kruus K, Saloheimo M, Paananen A, Koivula A, Rouvinen J, Nat Struct Biol. 2002 Aug;9(8):601-5. PMID:12118243
Page seeded by OCA on Tue Dec 18 15:40:51 2007
Categories: Laccase | Melanocarpus albomyces | Single protein | Hakulinen, N. | Kiiskinen, L.L. | Koivula, A. | Kruus, K. | Rouvinen, J. | Saloheimo, M. | CL | CU | NAG | OXY | SO4 | Ascomycete | C-terminal plug | Multi-copper oxidases | Oxygen reduction
