1shk
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1SHK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ | + | 1SHK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71]]. Structure known Active Sites: PIP, POP, SAS and SBS. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SHK OCA]]. |
==Reference== | ==Reference== | ||
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299895 15299895] | Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299895 15299895] | ||
[[Category: Erwinia chrysanthemi]] | [[Category: Erwinia chrysanthemi]] | ||
| + | [[Category: Shikimate kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Coggins, J.R.]] | [[Category: Coggins, J.R.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:30:40 2007'' |
Revision as of 08:25, 30 October 2007
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THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI
Overview
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia, coli has been crystallized by the vapour-diffusion method using sodium, chloride as a precipitant. Mass spectrometry was used to confirm the, purity of the shikimate kinase and dynamic light scattering was used to, assess conditions for the monodispersity of the enzyme. The crystals are, tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a, = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better, than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to, contain two molecules, corresponding to a packing density of 3.6 A(3), Da(-1).
About this Structure
1SHK is a [Single protein] structure of sequence from [Erwinia chrysanthemi] with MG as [ligand]. Active as [Shikimate kinase], with EC number [2.7.1.71]. Structure known Active Sites: PIP, POP, SAS and SBS. Full crystallographic information is available from [OCA].
Reference
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895
Page seeded by OCA on Tue Oct 30 10:30:40 2007
