1hj9
From Proteopedia
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| - | [[Image:1hj9. | + | [[Image:1hj9.jpg|left|200px]]<br /><applet load="1hj9" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1hj9" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1hj9, resolution 0.95Å" /> | caption="1hj9, resolution 0.95Å" /> | ||
'''ATOMIC RESOLUTION STRUCTURES OF TRYPSIN PROVIDE INSIGHT INTO STRUCTURAL RADIATION DAMAGE'''<br /> | '''ATOMIC RESOLUTION STRUCTURES OF TRYPSIN PROVIDE INSIGHT INTO STRUCTURAL RADIATION DAMAGE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HJ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4, CA, ANL and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] | + | 1HJ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4, CA, ANL and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Known structural/functional Site: <scene name='pdbsite=ANL:Ca Binding Site For Residue A1001'>ANL</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJ9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trypsin]] | [[Category: trypsin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:25:55 2007'' |
Revision as of 14:16, 18 December 2007
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ATOMIC RESOLUTION STRUCTURES OF TRYPSIN PROVIDE INSIGHT INTO STRUCTURAL RADIATION DAMAGE
Overview
Radiation damage is an inherent problem in protein X-ray crystallography, and the process has recently been shown to be highly specific, exhibiting, features such as cleavage of disulfide bonds, decarboxylation of acidic, residues, increase in atomic B factors and increase in unit-cell volume., Reported here are two trypsin structures at atomic resolution (1.00 and, 0.95 A), the data for which were collected at a third-generation, synchrotron (ESRF) at two different beamlines. Both trypsin structures, exhibit broken disulfide bonds; in particular, the bond from Cys191 to, Cys220 is very sensitive to synchrotron radiation. The data set collected, at the most intense beamline (ID14-EH4) shows increased structural, radiation damage in terms of lower occupancies for cysteine residues, more, breakage in the six disulfide bonds and more alternate conformations. It, appears that high intensity and not only the total X-ray dose is most, harmful to protein crystals.
About this Structure
1HJ9 is a Single protein structure of sequence from Bos taurus with SO4, CA, ANL and GOL as ligands. Active as Trypsin, with EC number 3.4.21.4 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Atomic resolution structures of trypsin provide insight into structural radiation damage., Leiros HK, McSweeney SM, Smalas AO, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:11264577
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