1kev
From Proteopedia
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| - | [[Image:1kev.gif|left|200px]]<br /> | + | [[Image:1kev.gif|left|200px]]<br /><applet load="1kev" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1kev" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1kev, resolution 2.05Å" /> | caption="1kev, resolution 2.05Å" /> | ||
'''STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE'''<br /> | '''STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KEV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_beijerinckii Clostridium beijerinckii] with ZN and NDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(NADP(+)) Alcohol dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.2 1.1.1.2] | + | 1KEV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_beijerinckii Clostridium beijerinckii] with ZN and NDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(NADP(+)) Alcohol dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.2 1.1.1.2] Known structural/functional Sites: <scene name='pdbsite=S1:Catalytic Site'>S1</scene>, <scene name='pdbsite=S2:Catalytic Site'>S2</scene>, <scene name='pdbsite=S3:Catalytic Site'>S3</scene> and <scene name='pdbsite=S4:Catalytic Site'>S4</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KEV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:41:01 2007'' |
Revision as of 14:31, 18 December 2007
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STRUCTURE OF NADP-DEPENDENT ALCOHOL DEHYDROGENASE
Overview
Two tetrameric NADP(+)-dependent bacterial secondary alcohol, dehydrogenases have been crystallized in the apo- and the holo-enzyme, forms. Crystals of the holo-enzyme from the mesophilic Clostridium, beijerinckii (NCBAD) belong to space group P2(1)2(1)2(1) with unit-cell, dimensions a = 90.5, b = 127.9, c = 151.4 A. Crystals of the apo-enzyme, (CBAD) belong to the same space group with unit-cell dimensions a = 80.4, b = 102.3, c = 193.5 A. Crystals of the holo-enzyme from the thermophilic, Thermoanaerobium brockii (NTBAD) belong to space group P6(1(5)) (a = b =, 80.6, c = 400.7 A). Crystals of the apo-form of TBAD (point mutant GI98D), belong to space group P2(1) with cell dimensions a = 123.0, b = 84.8, c =, 160.4 A beta = 99.5 degrees. Crystals of CBAD, NCBAD and NTBAD contain one, tetramer per asymmetric unit. They diffract to 2.0 A resolution at liquid, nitrogen temperature. Crystals of TBAD(GI98D) have two tetramers per, asymmetric unit and diffract to 2.7 A at 276 K. Self-rotation analysis, shows that both enzymes are tetramers of 222 symmetry.
About this Structure
1KEV is a Single protein structure of sequence from Clostridium beijerinckii with ZN and NDP as ligands. Active as Alcohol dehydrogenase (NADP(+)), with EC number 1.1.1.2 Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry., Korkhin Y, Frolow F, Bogin O, Peretz M, Kalb AJ, Burstein Y, Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):882-6. PMID:15299659
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