This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1nht
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1nht. | + | [[Image:1nht.jpg|left|200px]]<br /><applet load="1nht" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1nht" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1nht, resolution 2.5Å" /> | caption="1nht, resolution 2.5Å" /> | ||
'''ENTRAPMENT OF 6-THIOPHOSPHORYL-IMP IN THE ACTIVE SITE OF CRYSTALLINE ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI DATA COLLECTED AT 100K'''<br /> | '''ENTRAPMENT OF 6-THIOPHOSPHORYL-IMP IN THE ACTIVE SITE OF CRYSTALLINE ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI DATA COLLECTED AT 100K'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1NHT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, GDP, HAD and PGS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] | + | 1NHT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, GDP, HAD and PGS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] Known structural/functional Sites: <scene name='pdbsite=ASP:These Residues Make Up The Guanine Nucleotide Binding Si ...'>ASP</scene>, <scene name='pdbsite=GNS:These Residues Make Up The Guanine Nucleotide Binding Si ...'>GNS</scene> and <scene name='pdbsite=IMP:These Residues Make Up The Guanine Nucleotide Binding Si ...'>IMP</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NHT OCA]. |
==Reference== | ==Reference== | ||
| Line 29: | Line 28: | ||
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:49:02 2007'' |
Revision as of 14:39, 18 December 2007
|
ENTRAPMENT OF 6-THIOPHOSPHORYL-IMP IN THE ACTIVE SITE OF CRYSTALLINE ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI DATA COLLECTED AT 100K
Overview
Crystal structures of adenylosuccinate synthetase from Escherichia coli, complexed with Mg2+, 6-thiophosphoryl-IMP, GDP, and hadacidin at 298 and, 100 K have been refined to R-factors of 0.171 and 0.206 against data to, 2.8 and 2.5 A resolution, respectively. Interactions of GDP, Mg2+ and, hadacidin are similar to those observed for the same ligands in the, complex of IMP, GDP, NO3-, Mg2+ and hadacidin (Poland, B. W., Fromm, H. J., & Honzatko, R. B. (1996). J. Mol. Biol. 264, 1013-1027). Although crystals, were grown from solutions containing 6-mercapto-IMP and GTP, the electron, density at the active site is consistent with 6-thiophosphoryl-IMP and, GDP. Asp-13 and Gln-224 probably work in concert to stabilize the, 6-thioanion of 6-mercapto-IMP, which in turn is the nucleophile in the, displacement of GDP from the gamma-phosphate of GTP. Once formed, 6-thiophosphoryl-IMP is stable in the active site of the enzyme under the, conditions of the structural investigation. The direct observation of, 6-thiophosphoryl-IMP in the active site is consistent with the putative, generation of 6-phosphoryl-IMP along the reaction pathway of the, synthetase.
About this Structure
1NHT is a Single protein structure of sequence from Escherichia coli with MG, GDP, HAD and PGS as ligands. Active as Adenylosuccinate synthase, with EC number 6.3.4.4 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Entrapment of 6-thiophosphoryl-IMP in the active site of crystalline adenylosuccinate synthetase from Escherichia coli., Poland BW, Bruns C, Fromm HJ, Honzatko RB, J Biol Chem. 1997 Jun 13;272(24):15200-5. PMID:9182542
Page seeded by OCA on Tue Dec 18 16:49:02 2007
