1o97
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1o97. | + | [[Image:1o97.jpg|left|200px]]<br /><applet load="1o97" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1o97" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1o97, resolution 1.60Å" /> | caption="1o97, resolution 1.60Å" /> | ||
'''STRUCTURE OF ELECTRON TRANSFERRING FLAVOPROTEIN FROM METHYLOPHILUS METHYLOTROPHUS, RECOGNITION LOOP REMOVED BY LIMITED PROTEOLYSIS'''<br /> | '''STRUCTURE OF ELECTRON TRANSFERRING FLAVOPROTEIN FROM METHYLOPHILUS METHYLOTROPHUS, RECOGNITION LOOP REMOVED BY LIMITED PROTEOLYSIS'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1O97 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus] with AMP and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1O97 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus] with AMP and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AMP:Fad Binding Site For Chain D'>AMP</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O97 OCA]. |
==Reference== | ==Reference== | ||
| Line 25: | Line 24: | ||
[[Category: flavoprotein]] | [[Category: flavoprotein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:08:55 2007'' |
Revision as of 14:59, 18 December 2007
|
STRUCTURE OF ELECTRON TRANSFERRING FLAVOPROTEIN FROM METHYLOPHILUS METHYLOTROPHUS, RECOGNITION LOOP REMOVED BY LIMITED PROTEOLYSIS
Overview
Here we report the crystal structures of a ternary electron transfer, complex showing extensive motion at the protein interface. This, physiological complex comprises the iron-sulfur flavoprotein, trimethylamine dehydrogenase and electron transferring flavoprotein (ETF), from Methylophilus methylotrophus. In addition, we report the crystal, structure of free ETF. In the complex, electron density for the FAD domain, of ETF is absent, indicating high mobility. Positions for the FAD domain, are revealed by molecular dynamics simulation, consistent with crystal, structures and kinetic data. A dual interaction of ETF with trimethylamine, dehydrogenase provides for dynamical motion at the protein interface: one, site acts as an anchor, thereby allowing the other site to sample a large, range of interactions, some compatible with rapid electron transfer. This, study establishes the role of conformational sampling in multi-domain, redox systems, providing insight into electron transfer between ETFs and, structurally distinct redox partners.
About this Structure
1O97 is a Protein complex structure of sequences from Methylophilus methylotrophus with AMP and FAD as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Extensive conformational sampling in a ternary electron transfer complex., Leys D, Basran J, Talfournier F, Sutcliffe MJ, Scrutton NS, Nat Struct Biol. 2003 Mar;10(3):219-25. PMID:12567183
Page seeded by OCA on Tue Dec 18 17:08:55 2007
