This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4lip
From Proteopedia
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 4LIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]] with CA and CCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ | + | 4LIP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]] with CA and CCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Structure known Active Sites: ACD, ACE, OXD and OXE. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4LIP OCA]]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Burkholderia cepacia]] | [[Category: Burkholderia cepacia]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Triacylglycerol lipase]] | ||
[[Category: Dijkstra, B.W.]] | [[Category: Dijkstra, B.W.]] | ||
[[Category: Lang, D.A.]] | [[Category: Lang, D.A.]] | ||
| Line 25: | Line 26: | ||
[[Category: triglyceride analogue]] | [[Category: triglyceride analogue]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:33:50 2007'' |
Revision as of 08:29, 30 October 2007
|
PSEUDOMONAS LIPASE COMPLEXED WITH RC-(RP, SP)-DIBUTYLCARBAMOYLGLYCERO-3-O-BUTYLPHOSPHONATE
Overview
To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with Sc- and, Rc-(Rp,Sp)-1,2-dialkylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates, of different alkyl chain lengths. P. cepacia lipase was most rapidly, inactivated by Rc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl, octylphosphonate (Rc-trioctyl) with an inactivation half-time of 75 min, while that for the, Sc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl octyl-phosphonate, (Sc-trioctyl) compound was 530 min. X-ray structures were obtained of P., cepacia lipase after reaction with Rc-trioctyl to 0.29-nm resolution at pH, 4 and covalently modified with, Rc-(Rp,Sp)-1,2-dibutylcarbamoylglycero-3-O-p-nitrophenyl butyl-phosphonate, (Rc-tributyl) to 0.175-nm resolution ... [(full description)]
About this Structure
4LIP is a [Single protein] structure of sequence from [Burkholderia cepacia] with CA and CCP as [ligands]. Active as [Triacylglycerol lipase], with EC number [3.1.1.3]. Structure known Active Sites: ACD, ACE, OXD and OXE. Full crystallographic information is available from [OCA].
Reference
Structural basis of the chiral selectivity of Pseudomonas cepacia lipase., Lang DA, Mannesse ML, de Haas GH, Verheij HM, Dijkstra BW, Eur J Biochem. 1998 Jun 1;254(2):333-40. PMID:9660188
Page seeded by OCA on Tue Oct 30 10:33:50 2007
