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1qko
From Proteopedia
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| - | [[Image:1qko. | + | [[Image:1qko.jpg|left|200px]]<br /><applet load="1qko" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1qko" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1qko, resolution 2.10Å" /> | caption="1qko, resolution 2.10Å" /> | ||
'''HIGH RESOLUTION X-RAY STRUCTURE OF AN EARLY INTERMEDIATE IN THE BACTERIORHODOPSIN PHOTOCYCLE'''<br /> | '''HIGH RESOLUTION X-RAY STRUCTURE OF AN EARLY INTERMEDIATE IN THE BACTERIORHODOPSIN PHOTOCYCLE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QKO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum] with RET as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1QKO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum] with RET as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=SFF:Schiff Base'>SFF</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QKO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: retinal protein]] | [[Category: retinal protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:55:06 2007'' |
Revision as of 15:45, 18 December 2007
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HIGH RESOLUTION X-RAY STRUCTURE OF AN EARLY INTERMEDIATE IN THE BACTERIORHODOPSIN PHOTOCYCLE
Overview
Bacteriorhodopsin is the simplest known photon-driven proton pump and as, such provides a model for the study of a basic function in bioenergetics., Its seven transmembrane helices encompass a proton translocation pathway, containing the chromophore, a retinal molecule covalently bound to lysine, 216 through a protonated Schiff base, and a series of proton donors and, acceptors. Photoisomerization of the all-trans retinal to the 13-cis, configuration initiates the vectorial translocation of a proton from the, Schiff base, the primary proton donor, to the extracellular side, followed, by reprotonation of the Schiff base from the cytoplasm. Here we describe, the high-resolution X-ray structure of an early intermediate in the, photocycle of bacteriorhodopsin, which is formed directly after, photoexcitation. A key water molecule is dislocated, allowing the primary, proton acceptor, Asp 85, to move. Movement of the main-chain Lys 216, locally disrupts the hydrogen-bonding network of helix G, facilitating, structural changes later in the photocycle.
About this Structure
1QKO is a Single protein structure of sequence from Halobacterium salinarum with RET as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle., Edman K, Nollert P, Royant A, Belrhali H, Pebay-Peyroula E, Hajdu J, Neutze R, Landau EM, Nature. 1999 Oct 21;401(6755):822-6. PMID:10548112
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