1shk
From Proteopedia
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| - | [[Image:1shk.gif|left|200px]]<br /> | + | [[Image:1shk.gif|left|200px]]<br /><applet load="1shk" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1shk" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1shk, resolution 1.9Å" /> | caption="1shk, resolution 1.9Å" /> | ||
'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''<br /> | '''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SHK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] | + | 1SHK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] Known structural/functional Sites: <scene name='pdbsite=PIP:A Walker Type A Motif Forms The P-Loop Which Is The Bind ...'>PIP</scene>, <scene name='pdbsite=POP:A Walker Type A Motif Forms The P-Loop Which Is The Bind ...'>POP</scene>, <scene name='pdbsite=SAS:The Electron Density For Shikimate Was Ambiguous Prevent ...'>SAS</scene> and <scene name='pdbsite=SBS:The Electron Density For Shikimate Was Ambiguous Prevent ...'>SBS</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SHK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:00:08 2007'' |
Revision as of 15:50, 18 December 2007
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THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI
Overview
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia, coli has been crystallized by the vapour-diffusion method using sodium, chloride as a precipitant. Mass spectrometry was used to confirm the, purity of the shikimate kinase and dynamic light scattering was used to, assess conditions for the monodispersity of the enzyme. The crystals are, tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a, = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better, than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to, contain two molecules, corresponding to a packing density of 3.6 A(3), Da(-1).
About this Structure
1SHK is a Single protein structure of sequence from Erwinia chrysanthemi with MG as ligand. Active as Shikimate kinase, with EC number 2.7.1.71 Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895
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