1tez
From Proteopedia
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| - | [[Image:1tez.gif|left|200px]]<br /> | + | [[Image:1tez.gif|left|200px]]<br /><applet load="1tez" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1tez" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1tez, resolution 1.80Å" /> | caption="1tez, resolution 1.80Å" /> | ||
'''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS'''<br /> | '''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with MG, FAD and HDF as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] | + | 1TEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with MG, FAD and HDF as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] Known structural/functional Site: <scene name='pdbsite=ACT:Upon Complexation w. The Cpd-Damage The Active Site Appa ...'>ACT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: photolyase; dna repair; light-driven electron transfer]] | [[Category: photolyase; dna repair; light-driven electron transfer]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:01:40 2007'' |
Revision as of 15:51, 18 December 2007
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COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS
Overview
DNA photolyases use light energy to repair DNA that comprises, ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine, dimers (CPDs). Here we report the crystal structure of a DNA photolyase, bound to duplex DNA that is bent by 50 degrees and comprises a synthetic, CPD lesion. This CPD lesion is flipped into the active site and split, there into two thymines by synchrotron radiation at 100 K. Although, photolyases catalyze blue light-driven CPD cleavage only above 200 K, this, structure apparently mimics a structural substate during light-driven DNA, repair in which back-flipping of the thymines into duplex DNA has not yet, taken place.
About this Structure
1TEZ is a Single protein structure of sequence from Synechococcus sp. with MG, FAD and HDF as ligands. Active as Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622
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