1thj
From Proteopedia
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| - | [[Image:1thj.gif|left|200px]]<br /> | + | [[Image:1thj.gif|left|200px]]<br /><applet load="1thj" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1thj" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1thj, resolution 2.8Å" /> | caption="1thj, resolution 2.8Å" /> | ||
'''CARBONIC ANHYDRASE FROM METHANOSARCINA'''<br /> | '''CARBONIC ANHYDRASE FROM METHANOSARCINA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1THJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1THJ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb49_1.html Carbonic Anhydrase]]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] | + | 1THJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1THJ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb49_1.html Carbonic Anhydrase]]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Known structural/functional Sites: <scene name='pdbsite=1:Active Site 1, Located Between A And C Chain'>1</scene>, <scene name='pdbsite=2:Active Site 2, Located Between B And A Chain'>2</scene> and <scene name='pdbsite=3:Active Site 3, Located Between C And B Chain'>3</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1THJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: lyase (oxo-acid)]] | [[Category: lyase (oxo-acid)]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:01:58 2007'' |
Revision as of 15:52, 18 December 2007
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CARBONIC ANHYDRASE FROM METHANOSARCINA
Overview
A carbonic anhydrase from the thermophilic archaeon Methanosarcina, thermophila that exhibits no significant sequence similarity to known, carbonic anhydrases has recently been characterized. Here we present the, structure of this enzyme, which adopts a left-handed parallel beta-helix, fold. This fold is of particular interest since it contains only, left-handed crossover connections between the parallel beta-strands, which, so far have been observed very infrequently. The active form of the enzyme, is a trimer with three zinc-containing active sites, each located at the, interface between two monomers. While the arrangement of active site, groups differs between this enzyme and the carbonic anhydrases from higher, vertebrates, there are structural similarities in the zinc coordination, environment, suggestive of convergent evolution dictated by the chemical, requirements for catalysis of the same reaction. Based on sequence, similarities, the structure of this enzyme is the prototype of a new class, of carbonic anhydrases with representatives in all three phylogenetic, domains of life.
About this Structure
1THJ is a Single protein structure of sequence from Methanosarcina thermophila with ZN as ligand. The following page contains interesting information on the relation of 1THJ with [Carbonic Anhydrase]. Active as Carbonate dehydratase, with EC number 4.2.1.1 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
A left-hand beta-helix revealed by the crystal structure of a carbonic anhydrase from the archaeon Methanosarcina thermophila., Kisker C, Schindelin H, Alber BE, Ferry JG, Rees DC, EMBO J. 1996 May 15;15(10):2323-30. PMID:8665839
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