1axs
From Proteopedia
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[[Category: oxy-cope]] | [[Category: oxy-cope]] | ||
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Revision as of 08:33, 30 October 2007
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MATURE OXY-COPE CATALYTIC ANTIBODY WITH HAPTEN
Overview
Antibody catalysis provides an opportunity to examine the evolution of, binding energy and its relation to catalytic function in a system that has, many parallels with natural enzymes. Here we report such a study involving, an antibody AZ-28 that catalyses an oxy-Cope rearrangement, a pericyclic, reaction that belongs to a well studied and widely used class of reactions, in organic chemistry. Immunization with transition state analogue 1, results in a germline-encoded antibody that catalyses the rearrangement of, hexadiene 2 to aldehyde 3 with a rate approaching that of a related, pericyclic reaction catalysed by the enzyme chorismate mutase. Affinity, maturation gives antibody AZ-28, which has six amino acid substitutions, one of which results in a decrease in catalytic rate. To ... [(full description)]
About this Structure
1AXS is a [Protein complex] structure of sequences from [Homo sapiens] with CD and HOP as [ligands]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
The interplay between binding energy and catalysis in the evolution of a catalytic antibody., Ulrich HD, Mundorff E, Santarsiero BD, Driggers EM, Stevens RC, Schultz PG, Nature. 1997 Sep 18;389(6648):271-5. PMID:9305839
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