1uwk
From Proteopedia
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| - | [[Image:1uwk. | + | [[Image:1uwk.jpg|left|200px]]<br /><applet load="1uwk" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1uwk" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1uwk, resolution 1.19Å" /> | caption="1uwk, resolution 1.19Å" /> | ||
'''THE HIGH RESOLUTION STRUCTURE OF UROCANATE HYDRATASE FROM PSEUDOMONAS PUTIDA IN COMPLEX WITH UROCANATE'''<br /> | '''THE HIGH RESOLUTION STRUCTURE OF UROCANATE HYDRATASE FROM PSEUDOMONAS PUTIDA IN COMPLEX WITH UROCANATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UWK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with NAD and URO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urocanate_hydratase Urocanate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.49 4.2.1.49] | + | 1UWK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with NAD and URO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urocanate_hydratase Urocanate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.49 4.2.1.49] Known structural/functional Site: <scene name='pdbsite=AC1:Uro Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UWK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: urocanase]] | [[Category: urocanase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:14:38 2007'' |
Revision as of 16:04, 18 December 2007
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THE HIGH RESOLUTION STRUCTURE OF UROCANATE HYDRATASE FROM PSEUDOMONAS PUTIDA IN COMPLEX WITH UROCANATE
Overview
Urocanase (EC 4.2.1.49) from Pseudomonas putida was crystallized after, removing one of the seven free thiol groups. The crystal structure was, solved by multiwavelength anomalous diffraction (MAD) using a, seleno-methionine derivative and then refined at 1.14 A resolution. The, enzyme is a symmetric homodimer of 2 x 557 amino acid residues with, tightly bound NAD+ cofactors. Each subunit consists of a typical, NAD-binding domain inserted into a larger core domain that forms the dimer, interface. The core domain has a novel chain fold and accommodates the, substrate urocanate in a surface depression. The NAD domain sits like a, lid on the core domain depression and points with the nicotinamide group, to the substrate. Substrate, nicotinamide and five water molecules are, completely sequestered in a cavity. Most likely, one of these water, molecules hydrates the substrate during catalysis. This cavity has to open, for substrate passage, which probably means lifting the NAD domain. The, observed atomic arrangement at the active center gives rise to a detailed, proposal for the catalytic mechanism that is consistent with published, chemical data. As expected, the variability of the residues involved is, low, as derived from a family of 58 proteins annotated as urocanases in, the data banks. However, one well-embedded member of this family showed a, significant deviation at the active center indicating an incorrect, annotation.
About this Structure
1UWK is a Single protein structure of sequence from Pseudomonas putida with NAD and URO as ligands. Active as Urocanate hydratase, with EC number 4.2.1.49 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and action of urocanase., Kessler D, Retey J, Schulz GE, J Mol Biol. 2004 Sep 3;342(1):183-94. PMID:15313616
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