1uzm
From Proteopedia
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| - | [[Image:1uzm.gif|left|200px]]<br /> | + | [[Image:1uzm.gif|left|200px]]<br /><applet load="1uzm" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1uzm" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1uzm, resolution 1.49Å" /> | caption="1uzm, resolution 1.49Å" /> | ||
'''MABA FROM MYCOBACTERIUM TUBERCULOSIS'''<br /> | '''MABA FROM MYCOBACTERIUM TUBERCULOSIS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UZM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with CS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] | + | 1UZM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with CS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Known structural/functional Site: <scene name='pdbsite=AC1:Cs Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:19:37 2007'' |
Revision as of 16:09, 18 December 2007
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MABA FROM MYCOBACTERIUM TUBERCULOSIS
Overview
The fatty acid elongation system FAS-II is involved in the biosynthesis of, mycolic acids, which are major and specific long-chain fatty acids of the, cell envelope of Mycobacterium tuberculosis and other mycobacteria, including Mycobacterium smegmatis. The protein MabA, also named FabG1, has, been shown recently to be part of FAS-II and to catalyse the, NADPH-specific reduction of long chain beta-ketoacyl derivatives. This, activity corresponds to the second step of an FAS-II elongation round., FAS-II is inhibited by the antituberculous drug isoniazid through the, inhibition of the 2-trans-enoyl-acyl carrier protein reductase InhA. Thus, the other enzymes making up this enzymatic complex represent potential, targets for designing new antituberculous drugs. The crystal structure of, the apo-form MabA was solved to 2.03 A resolution by molecular, replacement. MabA is tetrameric and shares the conserved fold of the, short-chain dehydrogenases/reductases (SDRs). However, it exhibits some, significant local rearrangements of the active-site loops in the absence, of a cofactor, particularly the beta5-alpha5 region carrying the unique, tryptophan residue, in agreement with previous fluorescence spectroscopy, data. A similar conformation has been observed in the beta-ketoacyl, reductase from Escherichia coli and the distantly related dehydratase. The, distinctive enzymatic and structural properties of MabA are discussed in, view of its crystal structure and that of related enzymes.
About this Structure
1UZM is a Single protein structure of sequence from Mycobacterium tuberculosis with CS as ligand. Active as [acyl-carrier-protein_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number 1.1.1.100 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of MabA from Mycobacterium tuberculosis, a reductase involved in long-chain fatty acid biosynthesis., Cohen-Gonsaud M, Ducasse S, Hoh F, Zerbib D, Labesse G, Quemard A, J Mol Biol. 2002 Jul 5;320(2):249-61. PMID:12079383 [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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