1w2w

From Proteopedia

Revision as of 16:18, 18 December 2007

CRYSTAL STRUCTURE OF YEAST YPR118W, A METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE RELATED TO REGULATORY EIF2B SUBUNITS

Overview

Ypr118w is a non-essential, low copy number gene product from, Saccharomyces cerevisiae. It belongs to the PFAM family PF01008, which, contains the alpha-, beta-, and delta-subunits of eukaryotic translation, initiation factor eIF2B, as well as proteins of unknown function from all, three kingdoms. Recently, one of those latter proteins from Bacillus, subtilis has been characterized as a 5-methylthioribose-1-phosphate, isomerase, an enzyme of the methionine salvage pathway. We report here the, crystal structure of Ypr118w, which reveals a dimeric protein with two, domains and a putative active site cleft. The C-terminal domain resembles, ribose-5-phosphate isomerase from Escherichia coli with a similar location, of the active site. In vivo, Ypr118w protein is required for yeast cells, to grow on methylthioadenosine in the absence of methionine, showing that, Ypr118w is involved in the methionine salvage pathway. The crystal, structure of Ypr118w reveals for the first time the fold of a PF01008, member and allows a deeper discussion of an enzyme of the methionine, salvage pathway, which has in the past attracted interest due to tumor, suppression and as a target of aniprotozoal drugs.

About this Structure

1W2W is a Protein complex structure of sequences from Saccharomyces cerevisiae with SO4 as ligand. Active as S-methyl-5-thioribose-1-phosphate isomerase, with EC number 5.3.1.23 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits., Bumann M, Djafarzadeh S, Oberholzer AE, Bigler P, Altmann M, Trachsel H, Baumann U, J Biol Chem. 2004 Aug 27;279(35):37087-94. Epub 2004 Jun 23. PMID:15215245

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