1w7z
From Proteopedia
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| - | [[Image:1w7z.gif|left|200px]]<br /> | + | [[Image:1w7z.gif|left|200px]]<br /><applet load="1w7z" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1w7z" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1w7z, resolution 1.67Å" /> | caption="1w7z, resolution 1.67Å" /> | ||
'''CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)'''<br /> | '''CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] with NA and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1W7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] with NA and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Fmt Binding Site For Chain F'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W7Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trypsin]] | [[Category: trypsin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:33:45 2007'' |
Revision as of 16:23, 18 December 2007
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CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)
Overview
The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the, family of squash inhibitors and is one of the strongest inhibitors known, for trypsin. The eight independent molecules of EETI-II in the crystal, structure reported here provide a good opportunity to test the hypothesis, that this small cystine-knot protein (knottin) is sufficiently rigid to be, used as a molecular scaffold for protein-engineering purposes. To extend, this test, the structures of two complexes of EETI-II with trypsin have, also been determined, one carrying a four-amino-acid mutation of EETI-II., The remarkable similarity of these structures confirms the rigidity of the, molecular framework and hence its suitability as a molecular scaffold.
About this Structure
1W7Z is a Single protein structure of sequence from Ecballium elaterium with NA and FMT as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold., Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759
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