1wa6
From Proteopedia
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| - | [[Image:1wa6. | + | [[Image:1wa6.jpg|left|200px]]<br /><applet load="1wa6" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1wa6" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1wa6, resolution 2.55Å" /> | caption="1wa6, resolution 2.55Å" /> | ||
'''THE STRUCTURE OF ACC OXIDASE'''<br /> | '''THE STRUCTURE OF ACC OXIDASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida] with FE2, PO4 and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aminocyclopropanecarboxylate_oxidase Aminocyclopropanecarboxylate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.17.4 1.14.17.4] | + | 1WA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida] with FE2, PO4 and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aminocyclopropanecarboxylate_oxidase Aminocyclopropanecarboxylate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.17.4 1.14.17.4] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain X'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WA6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: acco]] | [[Category: acco]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:36:11 2007'' |
Revision as of 16:26, 18 December 2007
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THE STRUCTURE OF ACC OXIDASE
Overview
The final step in the biosynthesis of the plant signaling molecule, ethylene is catalyzed by 1-aminocyclopropane-1-carboxylic acid oxidase, (ACCO). ACCO requires bicarbonate as an activator and catalyzes the, oxidation of ACC to give ethylene, CO2, and HCN. We report crystal, structures of ACCO in apo-form (2.1 A resolution) and complexed with, Fe(II) (2.55 A) or Co(II) (2.4 A). The active site contains a single, Fe(II) ligated by three residues (His177, Asp179, and His234), and it is, relatively open compared to those of the 2-oxoglutarate oxygenases. The, side chains of Arg175 and Arg244, proposed to be involved in binding, bicarbonate, project away from the active site, but conformational changes, may allow either or both to enter the active site. The structures will, form a basis for future mechanistic and inhibition studies.
About this Structure
1WA6 is a Single protein structure of sequence from Petunia x hybrida with FE2, PO4 and SO4 as ligands. Active as Aminocyclopropanecarboxylate oxidase, with EC number 1.14.17.4 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase--the ethylene-forming enzyme., Zhang Z, Ren JS, Clifton IJ, Schofield CJ, Chem Biol. 2004 Oct;11(10):1383-94. PMID:15489165
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