User:Jaime B. Hutchison/Sandbox 1
From Proteopedia
(Difference between revisions)
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<scene name='Sandbox_Reserved_126/Dimer/2'>4 Cys amino acids</scene>. | <scene name='Sandbox_Reserved_126/Dimer/2'>4 Cys amino acids</scene>. | ||
(<font color='orange'>'''Cys is orange'''</font>.) | (<font color='orange'>'''Cys is orange'''</font>.) | ||
| - | We would like to make fluorescent mutants for microscopy. One way to do this is by attaching maleimide dye to cysteine (Cys) amino acids present in the protein. First we need to check that there are | + | We would like to make fluorescent mutants for microscopy applications. One way to do this is by attaching maleimide dye to cysteine (Cys) amino acids present in the protein. First we need to check that there are |
<scene name='Sandbox_Reserved_126/Dimer/4'>cys sites available on the surface</scene>. | <scene name='Sandbox_Reserved_126/Dimer/4'>cys sites available on the surface</scene>. | ||
| - | + | Because Cys 82 looks to be accessible and because the placement of Cys 66 looks like attaching a fluorophore to it may interfere with membrane binding we will mutate | |
| - | <scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/1'>this Cys</scene> | + | <scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/1'>this Cys</scene>. |
Revision as of 13:18, 3 June 2010
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The dimer contains . (Cys is orange.) We would like to make fluorescent mutants for microscopy applications. One way to do this is by attaching maleimide dye to cysteine (Cys) amino acids present in the protein. First we need to check that there are . Because Cys 82 looks to be accessible and because the placement of Cys 66 looks like attaching a fluorophore to it may interfere with membrane binding we will mutate .
