User:Jaime B. Hutchison/Sandbox 1
From Proteopedia
(Difference between revisions)
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Because Cys 82 looks to be accessible and because the placement of Cys 66 looks like attaching a fluorophore to it may interfere with membrane binding we will mutate | Because Cys 82 looks to be accessible and because the placement of Cys 66 looks like attaching a fluorophore to it may interfere with membrane binding we will mutate | ||
<scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/1'>Cys 66</scene>. | <scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/1'>Cys 66</scene>. | ||
| - | This will be done for | + | This will be done for each monomer, |
| - | <scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/ | + | <scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/5'>Cys 66 in chain A and B</scene>. |
Revision as of 14:05, 3 June 2010
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The dimer contains . (Cys is orange.) We would like to make fluorescent mutants for microscopy applications. One way to do this is by attaching maleimide dye to Cys amino acids present in the protein. First we need to check that there are . Because Cys 82 looks to be accessible and because the placement of Cys 66 looks like attaching a fluorophore to it may interfere with membrane binding we will mutate . This will be done for each monomer, .
