User:Jaime B. Hutchison/Sandbox 1
From Proteopedia
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The mutation will replace the cysteines at residue 66 with | The mutation will replace the cysteines at residue 66 with | ||
<scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/6'>alanines (Ala)</scene>. | <scene name='User:Jaime_B._Hutchison/Sandbox_1/Dimer/6'>alanines (Ala)</scene>. | ||
| + | (<font color='purple'>'''Ala is purple'''</font>.) | ||
Revision as of 14:16, 3 June 2010
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The dimer contains . (Cys is orange.) We would like to make fluorescent mutants for microscopy applications. One way to do this is by attaching maleimide dye to Cys amino acids present in the protein. First we need to check that there are . Because Cys 82 looks to be accessible and because the placement of Cys 66 looks like attaching a fluorophore to it may interfere with membrane binding we will mutate . This will be done for each monomer, . The mutation will replace the cysteines at residue 66 with . (Ala is purple.)
