2ahj
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2ahj.gif|left|200px]]<br /> | + | [[Image:2ahj.gif|left|200px]]<br /><applet load="2ahj" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2ahj" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2ahj, resolution 1.7Å" /> | caption="2ahj, resolution 1.7Å" /> | ||
'''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE'''<br /> | '''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2AHJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis] with FE, ZN, SO4, NO and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] | + | 2AHJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis] with FE, ZN, SO4, NO and DIO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] Known structural/functional Sites: <scene name='pdbsite=1:In Chain C'>1</scene>, <scene name='pdbsite=CTA:Non-Heme Fe Center And Catalytic Site Site_description I ...'>CTA</scene> and <scene name='pdbsite=CTB:In Chain A Site_identifier Ctb Site_description Non-Heme ...'>CTB</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AHJ OCA]. |
==Reference== | ==Reference== | ||
| Line 36: | Line 35: | ||
[[Category: post-translational modification of cysteine residues]] | [[Category: post-translational modification of cysteine residues]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:43:20 2007'' |
Revision as of 16:33, 18 December 2007
|
NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE
Overview
The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme, that is inactivated in the dark because of persistent association with NO, and activated by photo-dissociation of NO. The crystal structure at 1.7 A, resolution and mass spectrometry revealed the structure of the non-heme, iron catalytic center in the nitrosylated state. Two Cys residues, coordinated to the iron were post-translationally modified to Cys-sulfenic, and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO, molecule. This unprecedented structure is likely to enable the, photo-regulation of NHase and will provide an excellent model for, designing photo-controllable chelate complexes and, ultimately, proteins.
About this Structure
2AHJ is a Protein complex structure of sequences from Rhodococcus erythropolis with FE, ZN, SO4, NO and DIO as ligands. Active as Nitrile hydratase, with EC number 4.2.1.84 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
Reference
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:9586994
Page seeded by OCA on Tue Dec 18 18:43:20 2007
Categories: Nitrile hydratase | Protein complex | Rhodococcus erythropolis | Dohmae, N. | Endo, I. | Kamiya, N. | Nagashima, S. | Nakasako, M. | Odaka, M. | Takio, K. | Tsujimura, M. | Yohda, M. | DIO | FE | NO | SO4 | ZN | Hydratase | Lyase | Nitric oxide binding enzyme | Non-heme iron center | Photoreactive enzyme | Post-translational modification of cysteine residues
