2bfy
From Proteopedia
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| - | [[Image:2bfy.gif|left|200px]]<br /> | + | [[Image:2bfy.gif|left|200px]]<br /><applet load="2bfy" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2bfy" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2bfy, resolution 1.80Å" /> | caption="2bfy, resolution 1.80Å" /> | ||
'''COMPLEX OF AURORA-B WITH INCENP AND HESPERIDIN.'''<br /> | '''COMPLEX OF AURORA-B WITH INCENP AND HESPERIDIN.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BFY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with H1N as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 2BFY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with H1N as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:H1n Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BFY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase complex]] | [[Category: transferase complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:45:34 2007'' |
Revision as of 16:35, 18 December 2007
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COMPLEX OF AURORA-B WITH INCENP AND HESPERIDIN.
Overview
Aurora family serine/threonine kinases control mitotic progression, and, their deregulation is implicated in tumorigenesis. Aurora A and Aurora B, the best-characterized members of mammalian Aurora kinases, are, approximately 60% identical but bind to unrelated activating subunits. The, structure of the complex of Aurora A with the TPX2 activator has been, reported previously. Here, we report the crystal structure of Aurora B in, complex with the IN-box segment of the inner centromere protein (INCENP), activator and with the small molecule inhibitor Hesperadin. The Aurora, B:INCENP complex is remarkably different from the Aurora A:TPX2 complex., INCENP forms a crown around the small lobe of Aurora B and induces the, active conformation of the T loop allosterically. The structure represents, an intermediate state of activation of Aurora B in which the Aurora B, C-terminal segment stabilizes an open conformation of the catalytic cleft, and a critical ion pair in the kinase active site is impaired., Phosphorylation of two serines in the carboxyl terminus of INCENP, generates the fully active kinase.
About this Structure
2BFY is a Protein complex structure of sequences from Xenopus laevis with H1N as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Mechanism of Aurora B activation by INCENP and inhibition by hesperadin., Sessa F, Mapelli M, Ciferri C, Tarricone C, Areces LB, Schneider TR, Stukenberg PT, Musacchio A, Mol Cell. 2005 Apr 29;18(3):379-91. PMID:15866179
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