2bhz
From Proteopedia
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| - | [[Image:2bhz.gif|left|200px]]<br /> | + | [[Image:2bhz.gif|left|200px]]<br /><applet load="2bhz" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2bhz" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2bhz, resolution 1.20Å" /> | caption="2bhz, resolution 1.20Å" /> | ||
'''CRYSTAL STRUCTURE OF DEINOCOCCUS RADIODURANS MALTOOLIGOSYLTREHALOSE TREHALOHYDROLASE IN COMPLEX WITH MALTOSE'''<br /> | '''CRYSTAL STRUCTURE OF DEINOCOCCUS RADIODURANS MALTOOLIGOSYLTREHALOSE TREHALOHYDROLASE IN COMPLEX WITH MALTOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BHZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with MAL, MG, BME and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] | + | 2BHZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with MAL, MG, BME and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trehalose]] | [[Category: trehalose]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:47:41 2007'' |
Revision as of 16:37, 18 December 2007
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CRYSTAL STRUCTURE OF DEINOCOCCUS RADIODURANS MALTOOLIGOSYLTREHALOSE TREHALOHYDROLASE IN COMPLEX WITH MALTOSE
Overview
Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranose) is a, non-reducing diglucoside found in various organisms that serves as a, carbohydrate reserve and as an agent that protects against a variety of, physical and chemical stresses. Deinococcus radiodurans possesses an, alternative biosynthesis pathway for the synthesis of trehalose from, maltooligosaccharides. This reaction is mediated by two enzymes:, maltooligosyltrehalose synthase (MTSase) and maltooligosyltrehalose, trehalohydrolase (MTHase). Here, we present the 1.1A resolution crystal, structure of MTHase. It consists of three major domains: two beta-sheet, domains and a conserved glycosidase (beta/alpha)8 barrel catalytic domain., Three subdomains consisting of short insertions were identified within the, catalytic domain. Subsequently, structures of MTHase in complex with, maltose and trehalose were obtained at 1.2 A and 1.5 A resolution, respectively. These structures reveal the importance of the three inserted, subdomains in providing the key residues required for substrate, recognition. Trehalose is recognised specifically in the +1 and +2 binding, subsites by an extensive hydrogen-bonding network and a strong hydrophobic, stacking interaction in between two aromatic residues. Moreover, upon, binding to maltose, which mimics the substrate sugar chain, a major, concerted conformational change traps the sugar chain in the active site., The presence of magnesium in the active site of the MTHase-maltose complex, suggests that MTHase activity may be regulated by divalent cations.
About this Structure
2BHZ is a Single protein structure of sequence from Deinococcus radiodurans with MAL, MG, BME and TRS as ligands. Active as Alpha-amylase, with EC number 3.2.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of maltooligosyltrehalose trehalohydrolase from Deinococcus radiodurans in complex with disaccharides., Timmins J, Leiros HK, Leonard G, Leiros I, McSweeney S, J Mol Biol. 2005 Apr 15;347(5):949-63. PMID:15784255
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