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2bku
From Proteopedia
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| - | [[Image:2bku.gif|left|200px]]<br /> | + | [[Image:2bku.gif|left|200px]]<br /><applet load="2bku" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2bku" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2bku, resolution 2.70Å" /> | caption="2bku, resolution 2.70Å" /> | ||
'''KAP95P:RANGTP COMPLEX'''<br /> | '''KAP95P:RANGTP COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BKU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2BKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. | + | 2BKU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2BKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BKU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rangtp]] | [[Category: rangtp]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:50:31 2007'' |
Revision as of 16:40, 18 December 2007
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KAP95P:RANGTP COMPLEX
Overview
Nuclear protein import is mediated mainly by the transport factor, importin-beta that binds cytoplasmic cargo, most often via the, importin-alpha adaptor, and then transports it through nuclear pore, complexes. This active transport is driven by disassembly of the import, complex by nuclear RanGTP. The switch I and II loops of Ran change, conformation with nucleotide state, and regulate its interactions with, nuclear trafficking components. Importin-beta consists of 19 HEAT repeats, that are based on a pair of antiparallel alpha-helices (referred to as the, A- and B-helices). The HEAT repeats stack to yield two C-shaped arches, linked together to form a helicoidal molecule that has considerable, conformational flexibility. Here we present the structure of full-length, yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides a basis for understanding the crucial cargo-release step of, nuclear import. We identify a key interaction site where the RanGTP switch, I loop binds to the carboxy-terminal arch of Kap95p. This interaction, produces a change in helicoidal pitch that locks Kap95p in a conformation, that cannot bind importin-alpha or cargo. We suggest an allosteric, mechanism for nuclear import complex disassembly by RanGTP.
About this Structure
2BKU is a Protein complex structure of sequences from Canis lupus familiaris and Saccharomyces cerevisiae with MG and GTP as ligands. The following page contains interesting information on the relation of 2BKU with [Importins]. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis for nuclear import complex dissociation by RanGTP., Lee SJ, Matsuura Y, Liu SM, Stewart M, Nature. 2005 Jun 2;435(7042):693-6. Epub 2005 May 1. PMID:15864302
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