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107d
From Proteopedia
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(New page: 200px<br /> <applet load="107d" size="450" color="white" frame="true" align="right" spinBox="true" caption="107d" /> '''SOLUTION STRUCTURE OF THE COVALENT DUOCARMY...)
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Revision as of 13:33, 29 October 2007
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SOLUTION STRUCTURE OF THE COVALENT DUOCARMYCIN A-DNA DUPLEX COMPLEX
Overview
Duocarmycin A is an antitumour antibiotic that binds covalently to the, minor groove N-3 position of adenine with sequence specificity for the, 3'-adenine in a d(A-A-A-A) tract in duplex DNA. The adenine ring becomes, protonated on duocarmycin adduct formation resulting in charge, delocalization over the purine ring system. We report on the solution, structure of duocarmycin A bound site specifically to A12 (designated, *A12+) in the sequence context d(T3-T4-T5-T6).d(A9-A10-A11-*A12+) within a, hairpin duplex. The solution structure was solved based on a combined, NMR-molecular dynamics study including NOE based intensity refinement. The, A and B-rings of duocarmycin are positioned deep within the walls of the, minor groove with the B-ring (which is furthest from the covalent linkage, ... [(full description)]
About this Structure
107D is a [Protein complex] structure of sequences from [[1]] with DUO as [ligand]. Full crystallographic information is available from [OCA].
Reference
Solution structure of the covalent duocarmycin A-DNA duplex complex., Lin CH, Patel DJ, J Mol Biol. 1995 Apr 21;248(1):162-79. PMID:7731041
Page seeded by OCA on Mon Oct 29 15:38:29 2007
Categories: Protein complex | Lin, C.H. | Patel, D.J. | DUO | Dna | Double helix | Nmr
