2bvn
From Proteopedia
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| - | [[Image:2bvn.gif|left|200px]]<br /> | + | [[Image:2bvn.gif|left|200px]]<br /><applet load="2bvn" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2bvn" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2bvn, resolution 2.30Å" /> | caption="2bvn, resolution 2.30Å" /> | ||
'''E. COLI EF-TU:GDPNP IN COMPLEX WITH THE ANTIBIOTIC ENACYLOXIN IIA'''<br /> | '''E. COLI EF-TU:GDPNP IN COMPLEX WITH THE ANTIBIOTIC ENACYLOXIN IIA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BVN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, GNP and ENX as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.5.3 Transferred entry: 3.6.5.3], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.48 3.6.1.48] | + | 2BVN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, GNP and ENX as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.5.3 Transferred entry: 3.6.5.3], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.48 3.6.1.48] Known structural/functional Site: <scene name='pdbsite=AC1:Enx Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BVN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: translation]] | [[Category: translation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:00:01 2007'' |
Revision as of 16:50, 18 December 2007
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E. COLI EF-TU:GDPNP IN COMPLEX WITH THE ANTIBIOTIC ENACYLOXIN IIA
Overview
Elongation factor (EF-) Tu.GTP is the carrier of aminoacyl-tRNA to the, programmed ribosome. Enacyloxin IIa inhibits bacterial protein synthesis, by hindering the release of EF-Tu.GDP from the ribosome. The crystal, structure of the Escherichia coli EF-Tu.guanylyl iminodiphosphate, (GDPNP).enacyloxin IIa complex at 2.3 A resolution presented here reveals, the location of the antibiotic at the interface of domains 1 and 3. The, binding site overlaps that of kirromycin, an antibiotic with a structure, that is unrelated to enacyloxin IIa but that also inhibits EF-Tu.GDP, release. As one of the major differences, the enacyloxin IIa tail borders, a hydrophobic pocket that is occupied by the longer tail of kirromycin, explaining the higher binding affinity of the latter., EF-Tu.GDPNP.enacyloxin IIa shows a disordered effector region that in the, Phe-tRNAPhe.EF-Tu (Thermus aquaticus).GDPNP.enacyloxin IIa complex, solved, at 3.1 A resolution, is stabilized by the interaction with tRNA. This work, clarifies the structural background of the action of enacyloxin IIa and, compares its properties with those of kirromycin, opening new perspectives, for structure-guided design of novel antibiotics.
About this Structure
2BVN is a Single protein structure of sequence from Escherichia coli with MG, GNP and ENX as ligands. Active as Transferred entry: 3.6.5.3, with EC number 3.6.1.48 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Enacyloxin IIa pinpoints a binding pocket of elongation factor Tu for development of novel antibiotics., Parmeggiani A, Krab IM, Watanabe T, Nielsen RC, Dahlberg C, Nyborg J, Nissen P, J Biol Chem. 2006 Feb 3;281(5):2893-900. Epub 2005 Oct 28. PMID:16257965
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