2by3
From Proteopedia
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| - | [[Image:2by3.gif|left|200px]]<br /> | + | [[Image:2by3.gif|left|200px]]<br /><applet load="2by3" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2by3" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2by3, resolution 1.50Å" /> | caption="2by3, resolution 1.50Å" /> | ||
'''IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION'''<br /> | '''IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BY3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with GLC, MG, TRS, TRE and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] | + | 2BY3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with GLC, MG, TRS, TRE and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BY3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: synchrotron radiation]] | [[Category: synchrotron radiation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:02:45 2007'' |
Revision as of 16:52, 18 December 2007
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IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION
Overview
This paper focuses on the radiation-damage effects when applying the same, total X-ray dose to protein crystals at different dose rates. These, experiments have been performed on both a selenomethionated protein and on, bovine trypsin using dose rates that span nearly two orders of magnitude., The results show no clear dose-rate effect on the global indicators of, radiation damage, but a small measurable dose-rate effect could be found, when studying specific radiation damage. It is hypothesized that this, observed dose-rate effect relates to differences in the steady-state, free-radical concentration.
About this Structure
2BY3 is a Single protein structure of sequence from Deinococcus radiodurans with GLC, MG, TRS, TRE and BME as ligands. Active as Alpha-amylase, with EC number 3.2.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442
Page seeded by OCA on Tue Dec 18 19:02:45 2007
Categories: Alpha-amylase | Deinococcus radiodurans | Single protein | Leiros, H.K.S. | Mcsweeney, S.M. | Ravelli, R.B.G. | Timmins, J. | BME | GLC | MG | TRE | TRS | Data collection | Dose-rate | Hydrolase | Radiation damage | Synchrotron radiation
