2c07
From Proteopedia
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| - | [[Image:2c07.gif|left|200px]]<br /> | + | [[Image:2c07.gif|left|200px]]<br /><applet load="2c07" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2c07" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2c07, resolution 1.50Å" /> | caption="2c07, resolution 1.50Å" /> | ||
'''OXOACYL-ACP REDUCTASE OF PLASMODIUM FALCIPARUM'''<br /> | '''OXOACYL-ACP REDUCTASE OF PLASMODIUM FALCIPARUM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] | + | 2C07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C07 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: short-chain alcohol reductase]] | [[Category: short-chain alcohol reductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:04:49 2007'' |
Revision as of 16:55, 18 December 2007
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OXOACYL-ACP REDUCTASE OF PLASMODIUM FALCIPARUM
Overview
Type II fatty acid biosynthesis represents an attractive target for the, discovery of new antimalarial drugs. Previous studies have identified, malarial ENR (enoyl acyl-carrier-protein reductase, or FabI) as the target, for the antiseptic triclosan. In the present paper, we report the, biochemical properties and 1.5 A (1 A=0.1 nm) crystal structure of OAR, (3-oxoacyl acyl-carrier-protein reductase, or FabG), the second reductive, step in fatty acid biosynthesis and its inhibition by hexachlorophene., Under optimal conditions of pH and ionic strength, Plasmodium falciparum, OAR displays kinetic properties similar to those of OAR from bacteria or, plants. Activity with NADH is <3% of that with NADPH. Fluorescence, enhancement studies indicate that NADPH can bind to the free enzyme, consistent with kinetic and product inhibition studies suggesting a, steady-state ordered mechanism. The crystal structure reveals a tetramer, with a sulphate ion bound in the cofactor-binding site such that the side, chains of the catalytic triad of serine, tyrosine and lysine are aligned, in an active conformation, as previously observed in the Escherichia coli, OAR-NADP+ complex. A cluster of positively charged residues is positioned, at the entrance to the active site, consistent with the proposed, recognition site for the physiological substrate (3-oxoacyl-acyl-carrier, protein) in E. coli OAR. The antibacterial and anthelminthic agent, hexachlorophene is a potent inhibitor of OAR (IC50 2.05 microM) displaying, non-linear competitive inhibition with respect to NADPH. Hexachlorophene, (EC50 6.2 microM) and analogues such as bithionol also have antimalarial, activity in vitro, suggesting they might be useful leads for further, development.
About this Structure
2C07 is a Single protein structure of sequence from Plasmodium falciparum with SO4 as ligand. Active as [acyl-carrier-protein_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number 1.1.1.100 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Kinetic, inhibition and structural studies on 3-oxoacyl-ACP reductase from Plasmodium falciparum, a key enzyme in fatty acid biosynthesis., Wickramasinghe SR, Inglis KA, Urch JE, Muller S, van Aalten DM, Fairlamb AH, Biochem J. 2006 Jan 15;393(Pt 2):447-57. PMID:16225460 [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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