2c5b
From Proteopedia
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| - | [[Image:2c5b. | + | [[Image:2c5b.jpg|left|200px]]<br /><applet load="2c5b" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2c5b" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2c5b, resolution 2.50Å" /> | caption="2c5b, resolution 2.50Å" /> | ||
'''X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.'''<br /> | '''X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya] with MET and 5F1 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] | + | 2C5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya] with MET and 5F1 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] Known structural/functional Site: <scene name='pdbsite=AC1:MET Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C5B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:10:26 2007'' |
Revision as of 17:00, 18 December 2007
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X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.
Overview
The fluorinase enzyme from Streptomyces cattleya displays an unusual, ability in biocatalysis in that it forms a C-F bond. We now report that, the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to, accommodate this substrate series. It emerges from this study that the, enzyme does not require a planar ribose conformation of the substrate to, catalyse C-F bond formation.
About this Structure
2C5B is a Single protein structure of sequence from Streptomyces cattleya with MET and 5F1 as ligands. Active as Adenosyl-fluoride synthase, with EC number 2.5.1.63 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
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