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108d

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(New page: 200px<br /> <applet load="108d" size="450" color="white" frame="true" align="right" spinBox="true" caption="108d" /> '''THE SOLUTION STRUCTURE OF A DNA COMPLEX WIT...)
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Revision as of 13:34, 29 October 2007

Image:108d.gif

108d

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THE SOLUTION STRUCTURE OF A DNA COMPLEX WITH THE FLUORESCENT BIS INTERCALATOR TOTO DETERMINED BY NMR SPECTROSCOPY

Overview

We have used two-dimensional 1H NMR spectroscopy to determine the solution, structure of the DNA oligonucleotide d(5'-CGCTAGCG-3')2 complexed with the, bis-intercalating dye, 1,1'-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene)bis[4-(3-methyl -2,3-, dihydrobenzo-1,3-thiazolyl-2-methylidene)qui nolinium] tetraiodide (TOTO)., The determination of the structure was based on total relaxation matrix, analysis of the NOESY cross-peak intensities using the program MARDIGRAS., Improved procedures to consider the experimental "noise" in NOESY spectra, during these calculations have been employed. The NOE-derived distance, restraints were applied in restrained molecular dynamics calculations., Twenty final structures each were generated for the TOTO complex from both, A-form and B-form dsDNA ... [(full description)]

About this Structure

108D is a [Protein complex] structure of sequences from [[1]] with TOT as [ligand]. Full crystallographic information is available from [OCA].

Reference

Solution structure of a DNA complex with the fluorescent bis-intercalator TOTO determined by NMR spectroscopy., Spielmann HP, Wemmer DE, Jacobsen JP, Biochemistry. 1995 Jul 11;34(27):8542-53. PMID:7612596

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