2cfo
From Proteopedia
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| - | [[Image:2cfo.gif|left|200px]]<br /> | + | [[Image:2cfo.gif|left|200px]]<br /><applet load="2cfo" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2cfo" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2cfo, resolution 2.45Å" /> | caption="2cfo, resolution 2.45Å" /> | ||
'''NON-DISCRIMINATING GLUTAMYL-TRNA SYNTHETASE FROM THERMOSYNECHOCOCCUS ELONGATUS IN COMPLEX WITH GLU'''<br /> | '''NON-DISCRIMINATING GLUTAMYL-TRNA SYNTHETASE FROM THERMOSYNECHOCOCCUS ELONGATUS IN COMPLEX WITH GLU'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CFO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus] with GLU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17] | + | 2CFO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus] with GLU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17] Known structural/functional Site: <scene name='pdbsite=AC1:GLU Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein biosynthesis]] | [[Category: protein biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:20:52 2007'' |
Revision as of 17:11, 18 December 2007
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NON-DISCRIMINATING GLUTAMYL-TRNA SYNTHETASE FROM THERMOSYNECHOCOCCUS ELONGATUS IN COMPLEX WITH GLU
Overview
Error-free protein biosynthesis is dependent on the reliable charging of, each tRNA with its cognate amino acid. Many bacteria, however, lack a, glutaminyl-tRNA synthetase. In these organisms, tRNA(Gln) is initially, mischarged with glutamate by a non-discriminating glutamyl-tRNA synthetase, (ND-GluRS). This enzyme thus charges both tRNA(Glu) and tRNA(Gln) with, glutamate. Discriminating GluRS (D-GluRS), found in some bacteria and all, eukaryotes, exclusively generates Glu-tRNA(Glu). Here we present the first, crystal structure of a non-discriminating GluRS from Thermosynechococcus, elongatus (ND-GluRS(Tel)) in complex with glutamate at a resolution of, 2.45 A. Structurally, the enzyme shares the overall architecture of the, discriminating GluRS from Thermus thermophilus (D-GluRS(Tth)). We confirm, experimentally that GluRS(Tel) is non-discriminating and present kinetic, parameters for synthesis of Glu-tRNA(Glu) and of Glu-tRNA(Gln). Anticodons, of tRNA(Glu) (34C/UUC36) and tRNA(Gln) (34C/UUG36) differ only in base 36., The pyrimidine base of C36 is specifically recognized in D-GluRS(Tth) by, the residue Arg358. In ND-GluRS(Tel) this arginine residue is replaced by, glycine (Gly366) presumably allowing both cytosine and the bulkier purine, base G36 of tRNA(Gln) to be tolerated. Most other ND-GluRS share this, structural feature, leading to relaxed substrate specificity.
About this Structure
2CFO is a Single protein structure of sequence from Synechococcus elongatus with GLU as ligand. Active as Glutamate--tRNA ligase, with EC number 6.1.1.17 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a non-discriminating glutamyl-tRNA synthetase., Schulze JO, Masoumi A, Nickel D, Jahn M, Jahn D, Schubert WD, Heinz DW, J Mol Biol. 2006 Sep 1;361(5):888-97. Epub 2006 Jul 5. PMID:16876193
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