2iyo
From Proteopedia
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| - | [[Image:2iyo.gif|left|200px]]<br /> | + | [[Image:2iyo.gif|left|200px]]<br /><applet load="2iyo" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2iyo" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2iyo, resolution 2.40Å" /> | caption="2iyo, resolution 2.40Å" /> | ||
'''STRUCTURAL CHARACTERIZATION OF A BACTERIAL 6PDH REVEALS ASPECTS OF SPECIFICITY, MECHANISM AND MODE OF INHIBITION'''<br /> | '''STRUCTURAL CHARACTERIZATION OF A BACTERIAL 6PDH REVEALS ASPECTS OF SPECIFICITY, MECHANISM AND MODE OF INHIBITION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IYO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with 6PG and CAC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphogluconate_dehydrogenase_(decarboxylating) Phosphogluconate dehydrogenase (decarboxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.44 1.1.1.44] | + | 2IYO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with 6PG and CAC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphogluconate_dehydrogenase_(decarboxylating) Phosphogluconate dehydrogenase (decarboxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.44 1.1.1.44] Known structural/functional Site: <scene name='pdbsite=AC1:Cac Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IYO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: pentose shunt]] | [[Category: pentose shunt]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:41:00 2007'' |
Revision as of 17:31, 18 December 2007
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STRUCTURAL CHARACTERIZATION OF A BACTERIAL 6PDH REVEALS ASPECTS OF SPECIFICITY, MECHANISM AND MODE OF INHIBITION
Overview
Crystal structures of recombinant Lactococcus lactis 6-phosphogluconate, dehydrogenase (LlPDH) in complex with substrate, cofactor, product and, inhibitors have been determined. LlPDH shares significant sequence, identity with the enzymes from sheep liver and the protozoan parasite, Trypanosoma brucei for which structures have been reported. Comparisons, indicate that the key residues in the active site are highly conserved, as, are the interactions with the cofactor and the product ribulose, 5-phosphate. However, there are differences in the conformation of the, substrate 6-phosphogluconate which may reflect distinct states relevant to, catalysis. Analysis of the complex formed with the potent inhibitor, 4-phospho-d-erythronohydroxamic acid, suggests that this molecule does, indeed mimic the high-energy intermediate state that it was designed to., The analysis also identified, as a contaminant by-product of the inhibitor, synthesis, 4-phospho-d-erythronamide, which binds in similar fashion., LlPDH can now serve as a model system for structure-based inhibitor design, targeting the enzyme from Trypanosoma species.
About this Structure
2IYO is a Single protein structure of sequence from Lactococcus lactis with 6PG and CAC as ligands. Active as Phosphogluconate dehydrogenase (decarboxylating), with EC number 1.1.1.44 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates., Sundaramoorthy R, Iulek J, Barrett MP, Bidet O, Ruda GF, Gilbert IH, Hunter WN, FEBS J. 2007 Jan;274(1):275-86. PMID:17222187
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