2j0s
From Proteopedia
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| - | [[Image:2j0s.gif|left|200px]]<br /> | + | [[Image:2j0s.gif|left|200px]]<br /><applet load="2j0s" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j0s" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2j0s, resolution 2.21Å" /> | caption="2j0s, resolution 2.21Å" /> | ||
'''THE CRYSTAL STRUCTURE OF THE EXON JUNCTION COMPLEX AT 2.2 A RESOLUTION'''<br /> | '''THE CRYSTAL STRUCTURE OF THE EXON JUNCTION COMPLEX AT 2.2 A RESOLUTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J0S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2J0S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Anp Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:43:18 2007'' |
Revision as of 17:33, 18 December 2007
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THE CRYSTAL STRUCTURE OF THE EXON JUNCTION COMPLEX AT 2.2 A RESOLUTION
Overview
The exon junction complex (EJC) plays a major role in posttranscriptional, regulation of mRNA in metazoa. The EJC is deposited onto mRNA during, splicing and is transported to the cytoplasm where it influences, translation, surveillance, and localization of the spliced mRNA. The, complex is formed by the association of four proteins (eIF4AIII, Barentsz, [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of, the EJC reveals how it stably locks onto mRNA. The DEAD-box protein, eIF4AIII encloses an ATP molecule and provides the binding sites for six, ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5', nucleotide. An intertwined network of interactions anchors Mago-Y14 and, Btz at the interface between the two domains of eIF4AIII, effectively, stabilizing the ATP bound state. Comparison with the structure of the, eIF4AIII-Btz subcomplex that we have also determined reveals that large, conformational changes are required upon EJC assembly and disassembly.
About this Structure
2J0S is a Protein complex structure of sequences from Homo sapiens with MG and ANP as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA., Bono F, Ebert J, Lorentzen E, Conti E, Cell. 2006 Aug 25;126(4):713-25. PMID:16923391
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Categories: Homo sapiens | Protein complex | Bono, F. | Conti, E. | Ebert, J. | Lorentzen, E. | ANP | MG | Acetylation | Alternative splicing | Atp-binding | Coiled coil | Dead-box helicase | Dna-binding | Ejc | Helicase | Hydrolase | Mrna processing | Mrna splicing | Mrna transport | Nonsense-mediated mrna decay | Nuclear protein | Nucleotide-binding | Phosphorylation | Rna-binding | Rrna processing | Transport
