2j1a
From Proteopedia
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| - | [[Image:2j1a.gif|left|200px]]<br /> | + | [[Image:2j1a.gif|left|200px]]<br /><applet load="2j1a" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j1a" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2j1a, resolution 1.49Å" /> | caption="2j1a, resolution 1.49Å" /> | ||
'''STRUCTURE OF CBM32 FROM CLOSTRIDIUM PERFRINGENS BETA-N-ACETYLHEXOSAMINIDASE GH84C IN COMPLEX WITH GALACTOSE'''<br /> | '''STRUCTURE OF CBM32 FROM CLOSTRIDIUM PERFRINGENS BETA-N-ACETYLHEXOSAMINIDASE GH84C IN COMPLEX WITH GALACTOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J1A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens] with GAL and CA as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2J1A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens] with GAL and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Ca Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J1A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-carbohydrate interaction]] | [[Category: protein-carbohydrate interaction]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:44:06 2007'' |
Revision as of 17:34, 18 December 2007
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STRUCTURE OF CBM32 FROM CLOSTRIDIUM PERFRINGENS BETA-N-ACETYLHEXOSAMINIDASE GH84C IN COMPLEX WITH GALACTOSE
Overview
Clostridium perfringens is a notable colonizer of the human, gastrointestinal tract. This bacterium is quite remarkable for a human, pathogen by the number of glycoside hydrolases found in its genome. The, modularity of these enzymes is striking as is the frequent occurrence of, modules having amino acid sequence identity with family 32, carbohydrate-binding modules (CBMs), often referred to as F5/8 domains., Here we report the properties of family 32 CBMs from a C. perfringens, N-acetyl-beta-hexosaminidase. Macroarray, UV difference, and isothermal, titration calorimetry binding studies indicate a preference for the, disaccharide LacNAc (beta-d-galactosyl-1,4-beta-d-N-acetylglucosamine)., The molecular details of the interaction of this CBM with galactose, LacNAc, and the type II blood group H-trisaccharide are revealed by x-ray, crystallographic studies at resolutions of 1.49, 2.4, and 2.3 A, respectively.
About this Structure
2J1A is a Single protein structure of sequence from Clostridium perfringens with GAL and CA as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The interaction of a carbohydrate-binding module from a Clostridium perfringens N-acetyl-beta-hexosaminidase with its carbohydrate receptor., Ficko-Blean E, Boraston AB, J Biol Chem. 2006 Dec 8;281(49):37748-57. Epub 2006 Sep 21. PMID:16990278
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