2j9l
From Proteopedia
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| - | [[Image:2j9l. | + | [[Image:2j9l.jpg|left|200px]]<br /><applet load="2j9l" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j9l" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2j9l, resolution 2.300Å" /> | caption="2j9l, resolution 2.300Å" /> | ||
'''CYTOPLASMIC DOMAIN OF THE HUMAN CHLORIDE TRANSPORTER CLC-5 IN COMPLEX WITH ATP'''<br /> | '''CYTOPLASMIC DOMAIN OF THE HUMAN CHLORIDE TRANSPORTER CLC-5 IN COMPLEX WITH ATP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J9L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2J9L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J9L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: voltage-gated channel]] | [[Category: voltage-gated channel]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:56:26 2007'' |
Revision as of 17:46, 18 December 2007
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CYTOPLASMIC DOMAIN OF THE HUMAN CHLORIDE TRANSPORTER CLC-5 IN COMPLEX WITH ATP
Contents |
Overview
The ubiquitous CBS domains, which are found as part of cytoplasmic domains, in the ClC family of chloride channels and transporters, have previously, been identified as building blocks for regulatory nucleotide-binding, sites. Here we report the structures of the cytoplasmic domain of the, human transporter ClC-5 in complex with ATP and ADP. The nucleotides bind, to a specific site in the protein. As determined by equilibrium dialysis, the affinities for ATP, ADP and AMP are in the high micromolar range., Point mutations that interfere with nucleotide binding change the, transport behavior of a ClC-5 mutant expressed in Xenopus laevis oocytes., Our results establish the structural and energetic basis for the, interaction of ClC-5 with nucleotides and provide a framework for future, investigations.
Disease
Known diseases associated with this structure: Dent disease OMIM:[300008], Hypophosphatemic rickets OMIM:[300008], Nephrolithiasis, type I OMIM:[300008], Proteinuria, low molecular weight, with hypercalciuric nephrocalcinosis OMIM:[300008]
About this Structure
2J9L is a Single protein structure of sequence from Homo sapiens with CL and ATP as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Nucleotide recognition by the cytoplasmic domain of the human chloride transporter ClC-5., Meyer S, Savaresi S, Forster IC, Dutzler R, Nat Struct Mol Biol. 2007 Jan;14(1):60-7. Epub 2006 Dec 31. PMID:17195847
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Categories: Homo sapiens | Single protein | Dutzler, R. | Forster, I.C. | Meyer, S. | Savaresi, S. | ATP | CL | Cbs domain | Chloride | Chloride channel | Chloride transporter | Clc | Disease mutation | Domain | Ion channel | Ion transport | Membrane | Transmembrane | Transport | Voltage-gated channel
