2jfu
From Proteopedia
Line 1: | Line 1: | ||
- | [[Image:2jfu. | + | [[Image:2jfu.jpg|left|200px]]<br /><applet load="2jfu" size="450" color="white" frame="true" align="right" spinBox="true" |
- | <applet load="2jfu" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2jfu, resolution 1.8Å" /> | caption="2jfu, resolution 1.8Å" /> | ||
'''CRYSTAL STRUCTURE OF ENTEROCOCCUS FAECIUM GLUTAMATE RACEMASE IN COMPLEX WITH PHOSPHATE'''<br /> | '''CRYSTAL STRUCTURE OF ENTEROCOCCUS FAECIUM GLUTAMATE RACEMASE IN COMPLEX WITH PHOSPHATE'''<br /> | ||
Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
- | 2JFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamate_racemase Glutamate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.3 5.1.1.3] | + | 2JFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamate_racemase Glutamate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.3 5.1.1.3] Known structural/functional Site: <scene name='pdbsite=AC1:Po4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JFU OCA]. |
==Reference== | ==Reference== | ||
Line 21: | Line 20: | ||
[[Category: peptidoglycan biosynthesis]] | [[Category: peptidoglycan biosynthesis]] | ||
- | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:07:03 2007'' |
Revision as of 17:57, 18 December 2007
|
CRYSTAL STRUCTURE OF ENTEROCOCCUS FAECIUM GLUTAMATE RACEMASE IN COMPLEX WITH PHOSPHATE
Overview
Glutamate racemase is an enzyme essential to the bacterial cell wall, biosynthesis pathway, and has therefore been considered as a target for, antibacterial drug discovery. We characterized the glutamate racemases of, several pathogenic bacteria using structural and biochemical approaches., Here we describe three distinct mechanisms of regulation for the family of, glutamate racemases: allosteric activation by metabolic precursors, kinetic regulation through substrate inhibition, and D-glutamate recycling, using a d-amino acid transaminase. In a search for selective inhibitors, we identified a series of uncompetitive inhibitors specifically targeting, Helicobacter pylori glutamate racemase that bind to a cryptic allosteric, site, and used these inhibitors to probe the mechanistic and dynamic, features of the enzyme. These structural, kinetic and mutational studies, provide insight into the physiological regulation of these essential, enzymes and provide a basis for designing narrow-spectrum antimicrobial, agents.
About this Structure
2JFU is a Single protein structure of sequence from Enterococcus faecium with PO4 as ligand. Active as Glutamate racemase, with EC number 5.1.1.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Exploitation of structural and regulatory diversity in glutamate racemases., Lundqvist T, Fisher SL, Kern G, Folmer RH, Xue Y, Newton DT, Keating TA, Alm RA, de Jonge BL, Nature. 2007 Jun 14;447(7146):817-22. PMID:17568739
Page seeded by OCA on Tue Dec 18 20:07:03 2007