2jho
From Proteopedia
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| - | [[Image:2jho. | + | [[Image:2jho.jpg|left|200px]]<br /><applet load="2jho" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2jho" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2jho, resolution 1.40Å" /> | caption="2jho, resolution 1.40Å" /> | ||
'''CYANOMET SPERM WHALE MYOGLOBIN AT 1.4A RESOLUTION'''<br /> | '''CYANOMET SPERM WHALE MYOGLOBIN AT 1.4A RESOLUTION'''<br /> | ||
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| + | ==Overview== | ||
| + | X-ray absorption spectroscopy is exquisitely sensitive to the coordination, geometry of an absorbing atom and therefore allows bond distances and, angles of the surrounding atomic cluster to be measured with atomic, resolution. By contrast, the accuracy and resolution of metalloprotein, active sites obtainable from x-ray crystallography are often insufficient, to analyze the electronic properties of the metals that are essential for, their biological functions. Here, we demonstrate that the combination of, both methods on the same metalloprotein single crystal yields a structural, model of the protein with exceptional active-site resolution. To this end, we have collected an x-ray diffraction data set to 1.4-A resolution and Fe, K-edge polarized x-ray absorption near edge structure (XANES) spectra on, the same cyanomet sperm whale myoglobin crystal. The XANES spectra were, quantitatively analyzed by using a method based on the multiple scattering, approach, which yielded Fe-heme structural parameters with, +/-(0.02-0.07)-A accuracy on the atomic distances and +/-7 degrees on the, Fe-CN angle. These XANES-derived parameters were subsequently used as, restraints in the crystal structure refinement. By combining XANES and, x-ray diffraction, we have obtained an cyanomet sperm whale myoglobin, structural model with a higher precision of the bond lengths and angles at, the active site than would have been possible with crystallographic, analysis alone. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JHO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CYN, SO4, ZN and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2JHO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CYN, SO4, ZN and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Hem Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Cyn Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:So4 Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Zn Binding Site For Chain A'>AC4</scene> and <scene name='pdbsite=AC5:Zn Binding Site For Chain A'>AC5</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JHO OCA]. |
| + | |||
| + | ==Reference== | ||
| + | X-ray structure analysis of a metalloprotein with enhanced active-site resolution using in situ x-ray absorption near edge structure spectroscopy., Arcovito A, Benfatto M, Cianci M, Hasnain SS, Nienhaus K, Nienhaus GU, Savino C, Strange RW, Vallone B, Della Longa S, Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6211-6. Epub 2007 Apr 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17404234 17404234] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arcovito, A.]] | [[Category: Arcovito, A.]] | ||
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[[Category: Cianci, M.]] | [[Category: Cianci, M.]] | ||
[[Category: Hasnain, S.S.]] | [[Category: Hasnain, S.S.]] | ||
| - | [[Category: Longa, S. | + | [[Category: Longa, S.Della.]] |
[[Category: Nienhaus, G.U.]] | [[Category: Nienhaus, G.U.]] | ||
[[Category: Nienhaus, K.]] | [[Category: Nienhaus, K.]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:09:47 2007'' |
Revision as of 17:59, 18 December 2007
|
CYANOMET SPERM WHALE MYOGLOBIN AT 1.4A RESOLUTION
Overview
X-ray absorption spectroscopy is exquisitely sensitive to the coordination, geometry of an absorbing atom and therefore allows bond distances and, angles of the surrounding atomic cluster to be measured with atomic, resolution. By contrast, the accuracy and resolution of metalloprotein, active sites obtainable from x-ray crystallography are often insufficient, to analyze the electronic properties of the metals that are essential for, their biological functions. Here, we demonstrate that the combination of, both methods on the same metalloprotein single crystal yields a structural, model of the protein with exceptional active-site resolution. To this end, we have collected an x-ray diffraction data set to 1.4-A resolution and Fe, K-edge polarized x-ray absorption near edge structure (XANES) spectra on, the same cyanomet sperm whale myoglobin crystal. The XANES spectra were, quantitatively analyzed by using a method based on the multiple scattering, approach, which yielded Fe-heme structural parameters with, +/-(0.02-0.07)-A accuracy on the atomic distances and +/-7 degrees on the, Fe-CN angle. These XANES-derived parameters were subsequently used as, restraints in the crystal structure refinement. By combining XANES and, x-ray diffraction, we have obtained an cyanomet sperm whale myoglobin, structural model with a higher precision of the bond lengths and angles at, the active site than would have been possible with crystallographic, analysis alone.
About this Structure
2JHO is a Single protein structure of sequence from [1] with CYN, SO4, ZN and HEM as ligands. Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.
Reference
X-ray structure analysis of a metalloprotein with enhanced active-site resolution using in situ x-ray absorption near edge structure spectroscopy., Arcovito A, Benfatto M, Cianci M, Hasnain SS, Nienhaus K, Nienhaus GU, Savino C, Strange RW, Vallone B, Della Longa S, Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6211-6. Epub 2007 Apr 2. PMID:17404234
Page seeded by OCA on Tue Dec 18 20:09:47 2007
Categories: Single protein | Arcovito, A. | Benfatto, M. | Cianci, M. | Hasnain, S.S. | Longa, S.Della. | Nienhaus, G.U. | Nienhaus, K. | Savino, C. | Strange, R.W. | Vallone, B. | CYN | HEM | SO4 | ZN | Heme | Iron | Metal-binding | Muscle protein | Oxygen transport | Transport
